Construct: ORF ccsbBroad304_00653
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012941.1_s304c1, BRDN0000461178
- Derived from:
- ccsbBroadEn_00653
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAT1 (2779)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00653
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2779 | GNAT1 | G protein subunit alpha tra... | NM_000172.4 | 100% | 100% | |
| 2 | human | 2779 | GNAT1 | G protein subunit alpha tra... | NM_144499.3 | 100% | 100% | |
| 3 | mouse | 14685 | Gnat1 | guanine nucleotide binding ... | NM_008140.2 | 89.3% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1119
- ORF length:
- 1050
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggctggg gccagtgctg aggagaagca ctccagggag ctggaaaaga 121 agctgaaaga ggacgctgag aaggatgctc gaaccgtgaa gctgctgctt ctgggtgccg 181 gtgagtccgg gaagagcacc atcgtcaagc agatgaagat tatccaccag gacgggtact 241 cgctggaaga gtgcctcgag tttatcgcca tcatctacgg caacacgttg cagtccatcc 301 tggccatcgt acgcgccatg accacactca acatccagta cggagactct gcacgccagg 361 acgacgcccg gaagctgatg cacatggcag acactatcga ggagggcacg atgcccaagg 421 agatgtcgga catcatccag cggctgtgga aggactccgg tatccaggcc tgttttgagc 481 gcgcctcgga gtaccagctc aacgactcgg cgggctacta cctctccgac ctggagcgcc 541 tggtaacccc gggctacgtg cccaccgagc aggacgtgct gcgctcgcga gtcaagacca 601 ctggcatcat cgagacgcag ttctccttca aggatctcaa cttccggatg ttcgatgtgg 661 gcgggcagcg ctcggagcgc aagaagtgga tccactgctt cgagggcgtg acctgcatca 721 tcttcatcgc ggcgctgagc gcctacgaca tggtgctagt ggaggacgac gaagtgaacc 781 gcatgcacga gagcctgcac ctgttcaaca gcatctgcaa ccaccgctac ttcgccacga 841 cgtccatcgt gctcttcctt aacaagaagg acgtcttctt cgagaagatc aagaaggcgc 901 acctcagcat ctgtttcccg gactacgatg gacccaacac ctacgaggac gccggcaact 961 acatcaaggt gcagttcctc gagctcaaca tgcggcgcga cgtgaaggag atctattccc 1021 acatgacgtg cgccaccgac acgcagaacg tcaaatttgt cttcgacgct gtcaccgaca 1081 tcatcatcaa ggagaacctc aaagactgtg gcctcttctt gccaactttc ttgtacaaag 1141 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1201 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta