Construct: ORF ccsbBroad304_00665
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002208.1_s304c1, BRDN0000405584
- Derived from:
- ccsbBroadEn_00665
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GOT1 (2805)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00665
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2805 | GOT1 | glutamic-oxaloacetic transa... | NM_002079.3 | 100% | 100% | |
| 2 | mouse | 14718 | Got1 | glutamic-oxaloacetic transa... | NM_010324.2 | 87% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1305
- ORF length:
- 1239
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggnatggc acctccgtca gtctttgccg aggttccgca ggcccagcct gtcctggtct 121 tcaagctcac tgccgacttc agggaggatc cggacccccg caaggtcaac ctgggagtgg 181 gagcatatcg cacggatgac tgccatccct gggttttgcc agtagtgaag aaagtggagc 241 agaagattgc taatgacaat agcctaaatc acgagtatct gccaatcctg ggcctggctg 301 agttccggag ctgtgcttct cgtcttgccc ttggggatga cagcccagca ctcaaggaga 361 agcgggtagg aggtgtgcaa tctttggggg gaacaggtgc acttcgaatt ggagctgatt 421 tcttagcgcg ttggtacaat ggaacaaaca acaagaacac acctgtctat gtgtcctcac 481 caacctggga gaatcacaat gctgtgtttt ccgctgctgg ttttaaagac attcggtcct 541 atcgctactg ggatgcagag aagagaggat tggacctcca gggcttcctg aatgatctgg 601 agaatgctcc tgagttctcc attgttgtcc tccacgcctg tgcacacaac ccaactggga 661 ttgacccaac tccggagcag tggaagcaga ttgcttctgt catgaagcac cggtttctgt 721 tccccttctt tgactcagcc tatcagggct tcgcatctgg aaacctggag agagatgcct 781 gggccattcg ctattttgtg tctgaaggct tcgagttctt ctgtgcccag tccttctcca 841 agaacttcgg gctctacaat gagagagtcg ggaatctgac tgtggttgga aaagaacctg 901 agagcatcct gcaagtcctt tcccagatgg agaagatcgt gcggattact tggtccaatc 961 cccccgccca gggagcacga attgtggcca gcaccctctc taaccctgag ctctttgagg 1021 aatggacagg taatgtgaag acaatggctg accggattct gaccatgaga tctgaactca 1081 gggcacgact agaagccctc aaaacccctg ggacctggaa ccacatcact gatcaaattg 1141 gcatgttcag cttcactggg ttgaacccca agcaggttga gtatctggtc aatgaaaagc 1201 acatctacct gctgccaagt ggtcgaatca acgtgagtgg cttaaccacc aaaaatctag 1261 attacgtggc cacctccatc catgaagcag tcaccaaaat ccagtgaaga actttcttgt 1321 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1381 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a