Construct: ORF ccsbBroad304_00688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003886.1_s304c1, BRDN0000402838
- Derived from:
- ccsbBroadEn_00688
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GRB2 (2885)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00688
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5032 | P2RY11 | purinergic receptor P2Y11 | NM_002566.5 | 54.6% | 16.3% | (many diffs) |
| 2 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001040664.3 | 24.5% | 8.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agccatcgcc aaatatgact tcaaagctac tgcagacgac gagctgagct 121 tcaaaagggg ggacatcctc aaggttttga acgaagaatg tgatcagaac tggtacaagg 181 cagagcttaa tggaaaagac ggcttcattc ccaagaacta catagaaatg aaaccacatc 241 cgtggttttt tggcaaaatc cccagagcca aggcagaaga aatgcttagc aTACGGCGCC 301 CTCGGGCGGG CCGTGCTACG CAGCCCAGGC ATGACTGTGG CCGAGAAGCT GCGTGTGGCA 361 GCGTTGGTGG CCAGTGGTGT GGCCCTCTAC GCCAGCTCCT ATGTGCCCTA CCACATCATG 421 CGGGTGCTCA ACGTGGATGC TCGGCGGCGC TGGAGCACCC GCTGCCCGAG CTTTGCAGAC 481 ATAGCCCAGG CCACAGCAGC CCTGGAGCTG GGGCCCTACG TGGGCTACCA GGTGATGCGG 541 GGCCTCATGC CCCTGGCCTT CTGTGTCCAC CCTCTACTCT ACATGGCCGC AGTGCCCAGC 601 CTGGGCTGCT GCTGCCGACA CTGCCCCGGC TACAGGGACA GCTGGAACCC AGAGGACGCC 661 AAGAGCACTG GCCAAGCCCT GCCCCTCAAT GCCACAGCCG CCCCTAAACC GTCAGAGCCC 721 CAGTCCCGTG AGCTGAGCCA ATGCCCAACT TTCTTGTACa aagtggttgg taagcctatc 781 cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc 841 gttaagtcga caatcaacct ctggatta