Construct: ORF ccsbBroad304_00718

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013149.1_s304c1, BRDN0000404030
Derived from:
ccsbBroadEn_00718
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
H2AZ1 (3015)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00718
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3015 H2AZ1 H2A.Z variant histone 1 NM_002106.4 100% 100%
2 human 94239 H2AZ2 H2A.Z variant histone 2 NM_012412.4 80.8% 97.6% (many diffs)
3 human 94239 H2AZ2 H2A.Z variant histone 2 NM_201436.2 64.5% 77.3% (many diffs)
4 human 94239 H2AZ2 H2A.Z variant histone 2 NM_201517.2 59% 68.7% (many diffs)
5 mouse 51788 H2afz H2A histone family, member Z NM_016750.3 94.7% 100% (many diffs)
6 mouse 51788 H2afz H2A histone family, member Z NM_001316995.1 78.1% 82% (many diffs)
7 mouse 666634 Gm8203 predicted pseudogene 8203 XR_105012.5 41% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
450
ORF length:
384
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tggcggtaag gctggaaagg actccggaaa ggccaagaca aaggcggttt
121cccgctcgca gagagccggc ttgcagttcc cagtgggccg tattcatcga cacctaaaat
181ctaggacgac cagtcatgga cgtgtgggcg cgactgccgc tgtgtacagc gcagccatcc
241tggagtacct caccgcagag gtacttgaac tggcaggaaa tgcatcaaaa gacttaaagg
301taaagcgtat tacccctcgt cacttgcaac ttgctattcg tggagatgaa gaattggatt
361ctctcatcaa ggctacaatt gctggtggtg gtgtcattcc acacatccac aaatctctga
421ttgggaagaa aggacaacag aagactgtct acccaacttt cttgtacaaa gtggttggta
481agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
541ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)