Transcript: Mouse XR_105012.5

PREDICTED: Mus musculus predicted pseudogene 8203 (Gm8203), misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Gm8203 (666634)
Length:
887
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_105012.5
NBCI Gene record:
Gm8203 (666634)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_105012.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423587 ATTCGAGTCTTAACCATATTT pLKO_005 666 3UTR 100% 15.000 7.500 Y H2afz n/a
2 TRCN0000418776 AGGATGCCTGGATTCCTTATT pLKO_005 496 3UTR 100% 13.200 6.600 Y H2afz n/a
3 TRCN0000093045 CGTCACTTGCAGCTTGCTATA pLKO.1 362 3UTR 100% 10.800 5.400 Y H2afz n/a
4 TRCN0000432382 GTACTTGAGTTGGCAGGAAAT pLKO_005 305 3UTR 100% 10.800 5.400 Y H2afz n/a
5 TRCN0000421931 GACTTAAAGGTAAAGCGTATC pLKO_005 335 3UTR 100% 6.000 3.000 Y H2afz n/a
6 TRCN0000072583 GCTTCAAAGAAGCTATTGATT pLKO.1 700 3UTR 100% 5.625 2.813 Y H2AZ1 n/a
7 TRCN0000291923 GCTTCAAAGAAGCTATTGATT pLKO_005 700 3UTR 100% 5.625 2.813 Y H2AZ1 n/a
8 TRCN0000093043 CCTTATTATCTCAGGACTCTA pLKO.1 510 3UTR 100% 4.950 2.475 Y H2afz n/a
9 TRCN0000093046 CGGGAAGAAAGGACAACAGAA pLKO.1 466 3UTR 100% 4.950 2.475 Y H2afz n/a
10 TRCN0000093044 CCGTATTCATCGACACCTGAA pLKO.1 202 3UTR 100% 4.050 2.025 Y H2afz n/a
11 TRCN0000072585 CGTGGAGATGAAGAATTGGAT pLKO.1 383 3UTR 100% 3.000 1.500 Y H2AZ1 n/a
12 TRCN0000291924 CGTGGAGATGAAGAATTGGAT pLKO_005 383 3UTR 100% 3.000 1.500 Y H2AZ1 n/a
13 TRCN0000093047 CGACACCTGAAATCTAGGACA pLKO.1 212 3UTR 100% 2.640 1.320 Y H2afz n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_105012.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00718 pDONR223 100% 41% None (many diffs) n/a
2 ccsbBroad304_00718 pLX_304 0% 41% V5 (many diffs) n/a
3 TRCN0000470922 GCGTCATGAGTAACACGTACTCTT pLX_317 84.9% 41% V5 (many diffs) n/a
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