Construct: ORF ccsbBroad304_00754
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006075.1_s304c1, BRDN0000402573
- Derived from:
- ccsbBroadEn_00754
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN2 (3151)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00754
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3151 | HMGN2 | high mobility group nucleos... | NM_005517.4 | 100% | 100% | |
| 2 | human | 10473 | HMGN4 | high mobility group nucleos... | NM_006353.3 | 86.2% | 86.6% | (many diffs) |
| 3 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010172.2 | 86.2% | 86.6% | (many diffs) |
| 4 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010173.2 | 86.2% | 86.6% | (many diffs) |
| 5 | human | 107986383 | LOC107986383 | uncharacterized LOC107986383 | XR_001742516.1 | 27.4% | (many diffs) | |
| 6 | human | 283651 | HMGN2P46 | high mobility group nucleos... | NR_022014.1 | 12.7% | (many diffs) | |
| 7 | mouse | 15331 | Hmgn2 | high mobility group nucleos... | NM_016957.3 | 93.3% | 96.6% | (many diffs) |
| 8 | mouse | 627375 | Gm6750 | predicted gene 6750 | XR_873085.1 | 58.7% | (many diffs) | |
| 9 | mouse | 100503799 | Gm7931 | predicted pseudogene 7931; ... | XR_868409.2 | 21.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 336
- ORF length:
- 270
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc caagagaaag gctgaagggg atgctaaggg agataaagca aaggtgaagg 121 acgaaccaca gagaagatcc gcgaggttgt ctgctaaacc tgctcctcca aagccagagc 181 ccaagcctaa aaaggcccct gcaaagaagg gagagaaggt acccaaaggg aaaaagggaa 241 aagctgatgc tggcaaggag gggaataacc ctgcagaaaa tggagatgcc aaaacagacc 301 aggcacagaa agctgaaggt gctggagatg ccaagtgccc aactttcttg tacaaagtgg 361 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 421 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta