Construct: ORF ccsbBroad304_00758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010101.1_s304c1, BRDN0000405907
- Derived from:
- ccsbBroadEn_00758
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGA1 (3159)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00758
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_001319078.2 | 100% | 100% | |
| 2 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_001319079.2 | 100% | 100% | |
| 3 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_001319081.2 | 100% | 100% | |
| 4 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_001319082.2 | 100% | 100% | |
| 5 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_145899.3 | 100% | 100% | |
| 6 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_145901.2 | 100% | 100% | |
| 7 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_001319080.2 | 99% | 99% | 133_134insAGA |
| 8 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_001319077.2 | 89.7% | 89.7% | 101_102ins33 |
| 9 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_002131.4 | 89.7% | 89.7% | 101_102ins33 |
| 10 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_145902.3 | 89.7% | 89.7% | 101_102ins33 |
| 11 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_145903.3 | 89.7% | 89.7% | 101_102ins33 |
| 12 | human | 3159 | HMGA1 | high mobility group AT-hook 1 | NM_145905.2 | 89.7% | 89.7% | 101_102ins33 |
| 13 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001025427.3 | 91.9% | 97.1% | (many diffs) |
| 14 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001039356.2 | 91.9% | 97.1% | (many diffs) |
| 15 | mouse | 111241 | Hmga1-rs1 | high mobility group AT-hook... | NM_001166476.1 | 91.9% | 97.1% | (many diffs) |
| 16 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166535.1 | 91.9% | 97.1% | (many diffs) |
| 17 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166536.1 | 91.9% | 97.1% | (many diffs) |
| 18 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_016660.3 | 91.9% | 97.1% | (many diffs) |
| 19 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166543.1 | 90.9% | 96.2% | (many diffs) |
| 20 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166544.1 | 90.9% | 96.2% | (many diffs) |
| 21 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166545.1 | 88.5% | 93.6% | (many diffs) |
| 22 | mouse | 111241 | Hmga1-rs1 | high mobility group AT-hook... | NM_001166477.1 | 82.5% | 86.9% | (many diffs) |
| 23 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166537.1 | 82.5% | 86.9% | (many diffs) |
| 24 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166539.1 | 82.5% | 86.9% | (many diffs) |
| 25 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166540.1 | 82.5% | 86.9% | (many diffs) |
| 26 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166541.1 | 82.5% | 86.9% | (many diffs) |
| 27 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166542.1 | 82.5% | 86.9% | (many diffs) |
| 28 | mouse | 15361 | Hmga1 | high mobility group AT-hook 1 | NM_001166546.1 | 81.5% | 66.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 387
- ORF length:
- 321
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tgagtcgagc tcgaagtcca gccagccctt ggcctccaag caggaaaagg 121 acggcactga gaagcggggc cggggcaggc cgcgcaagca gcctccggtg agtcccggga 181 cagcgctggt agggagtcag aaggagccca gcgaagtgcc aacacctaag agacctcggg 241 gccgaccaaa gggaagcaaa aacaagggtg ctgccaagac ccggaaaacc accacaactc 301 caggaaggaa accaaggggc agacccaaaa aactggagaa ggaggaagag gagggcatct 361 cgcaggagtc ctcggaggag gagcagtgcc caactttctt gtacaaagtg gttggtaagc 421 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 481 ggcgcgttaa gtcgacaatc aacctctgga tta