Construct: ORF ccsbBroad304_00766
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009576.1_s304c1, BRDN0000403027
- Derived from:
- ccsbBroadEn_00766
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HNRNPD (3184)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00766
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3184 | HNRNPD | heterogeneous nuclear ribon... | NM_031370.3 | 100% | 100% | |
| 2 | human | 3184 | HNRNPD | heterogeneous nuclear ribon... | NM_031369.2 | 94.6% | 94.6% | 232_233ins57 |
| 3 | human | 3184 | HNRNPD | heterogeneous nuclear ribon... | NM_002138.3 | 86.1% | 85.9% | 853_854ins147 |
| 4 | human | 3184 | HNRNPD | heterogeneous nuclear ribon... | NM_001003810.1 | 80.8% | 80.5% | 232_233ins57;796_797ins147 |
| 5 | mouse | 11991 | Hnrnpd | heterogeneous nuclear ribon... | NM_001077265.2 | 94.7% | 97.1% | (many diffs) |
| 6 | mouse | 11991 | Hnrnpd | heterogeneous nuclear ribon... | XM_006534735.3 | 94.7% | 97.1% | (many diffs) |
| 7 | mouse | 11991 | Hnrnpd | heterogeneous nuclear ribon... | NM_001077266.2 | 89.6% | 92.4% | (many diffs) |
| 8 | mouse | 11991 | Hnrnpd | heterogeneous nuclear ribon... | NM_007516.3 | 81.1% | 83.1% | (many diffs) |
| 9 | mouse | 11991 | Hnrnpd | heterogeneous nuclear ribon... | NM_001077267.2 | 75.9% | 78.3% | (many diffs) |
| 10 | mouse | 11991 | Hnrnpd | heterogeneous nuclear ribon... | XR_389257.3 | 12.4% | (many diffs) | |
| 11 | mouse | 11991 | Hnrnpd | heterogeneous nuclear ribon... | XR_389258.3 | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1131
- ORF length:
- 1065
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggaggagcag ttcggcgggg acggggcggc ggcagcggca acggcggcgg 121 taggcggctc ggcgggcgag caggagggag ccatggtggc ggcgacacag ggggcagcgg 181 cggcggcggg aagcggagcc gggaccgggg gcggaaccgc gtctggaggc accgaagggg 241 gcagcgccga gtcggagggg gcgaagattg acgccagtaa gaacgaggag gatgaaggcc 301 attcaaactc ctccccacga cactctgaag cagcgacggc acagcgggaa gaatggaaaa 361 tgtttatagg aggccttagc tgggacacta caaagaaaga tctgaaggac tacttttcca 421 aatttggtga agttgtagac tgcactctga agttagatcc tatcacaggg cgatcaaggg 481 gttttggctt tgtgctattt aaagaatcgg agagtgtaga taaggtcatg gatcaaaaag 541 aacataaatt gaatgggaag gtgattgatc ctaaaagggc caaagccatg aaaacaaaag 601 agccggttaa aaaaattttt gttggtggcc tttctccaga tacacctgaa gagaaaataa 661 gggagtactt tggtggtttt ggtgaggtgg aatccataga gctccccatg gacaacaaga 721 ccaataagag gcgtgggttc tgctttatta cctttaagga agaagaacca gtgaagaaga 781 taatggaaaa gaaataccac aatgttggtc ttagtaaatg tgaaataaaa gtagccatgt 841 cgaaggaaca atatcagcaa cagcaacagt ggggatctag aggaggattt gcaggaagag 901 ctcgtggaag aggtggtggc cccagtcaaa actggaacca gggatatagt aactattgga 961 atcaaggcta tggcaactat ggatataaca gccaaggtta cggtggttat ggaggatatg 1021 actacactgg ttacaacaac tactatggat atggtgatta tagcaaccag cagagtggtt 1081 atgggaaggt atccaggcga ggtggtcatc aaaatagcta caaaccatac taaataactt 1141 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1201 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta