Construct: ORF ccsbBroad304_00781

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003902.1_s304c1, BRDN0000411076
Derived from:
ccsbBroadEn_00781
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HPD (3242)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00781
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3242 HPD 4-hydroxyphenylpyruvate dio... NM_002150.3 99.9% 99.7% 97A>G
2 human 3242 HPD 4-hydroxyphenylpyruvate dio... NM_001171993.1 90% 90% 0_1ins117
3 mouse 15445 Hpd 4-hydroxyphenylpyruvic acid... NM_008277.3 86% 89.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1245
ORF length:
1179
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac gacttacagt gacaaagggg caaagcctga gagaggccga ttcctccact
121tccactctgt gaccttctgg gttggcaacg ccaagcaggc cgcgtcattc tactgcagca
181agatgggctt tgaacctcta gcctacaggg gcctggagac cggttcccgg gaggtggtca
241gccatgtaat caaacaaggg aagattgtgt ttgtcctctc ctcagcgctc aacccctgga
301acaaagagat gggcgatcac ctggtgaaac acggtgacgg agtgaaggac attgcgttcg
361aggtggaaga ttgtgactac atcgtgcaga aagcacggga acggggcgcc aaaatcatgc
421gggagccctg ggtagagcaa gacaagtttg ggaaggtgaa gtttgctgtg ctgcagacgt
481atggggacac cacacacacc ctggtggaga agatgaacta catcggccaa ttcttgcctg
541gatatgaggc cccagcgttc atggaccccc tacttcctaa actgcccaaa tgcagtctgg
601agatgatcga ccacattgtg ggaaaccagc ctgatcagga gatggtgtcc gcctccgaat
661ggtacctgaa aaacctgcag ttccaccgct tctggtccgt ggatgacacg caggtgcaca
721cggaatatag ctctctgcga tccattgtgg tggccaacta tgaagagtcc atcaagatgc
781ccatcaatga gccagcgcct ggcaagaaga agtcccagat ccaggaatat gtggactata
841acgggggcgc tggggtccag cacatcgctc tcaagaccga agacatcatc acagcgattc
901gccacttgag agagagaggc ctggagttct tatctgttcc ctccacgtac tacaaacaac
961tgcgggagaa gctgaagacg gccaagatca aggtgaagga gaacattgat gccctggagg
1021agctgaaaat cctggtggac tacgacgaga aaggctacct cctgcagatc ttcaccaaac
1081cggtgcagga ccggcccacg ctcttcctgg aagtcatcca gcgccacaac caccagggtt
1141ttggagccgg caacttcaac tcactgttca aggctttcga ggaggagcag aacctgcggg
1201gtaacctcac caacatggag accaatgggg tggtgcccgg catgtaccca actttcttgt
1261acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1321gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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