Construct: ORF ccsbBroad304_00852
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016775.1_s304c1, BRDN0000407826
- Derived from:
- ccsbBroadEn_00852
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL7 (3574)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00852
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3574 | IL7 | interleukin 7 | NM_000880.4 | 100% | 100% | |
| 2 | human | 3574 | IL7 | interleukin 7 | NM_001199887.1 | 89.8% | 89.8% | 359_360ins54 |
| 3 | human | 3574 | IL7 | interleukin 7 | XM_011517522.3 | 79.1% | 74.8% | (many diffs) |
| 4 | human | 3574 | IL7 | interleukin 7 | XM_011517523.3 | 78.2% | 78.5% | 414_415insGA;417_422delTCTCGT;426_427ins109 |
| 5 | human | 3574 | IL7 | interleukin 7 | NM_001199886.1 | 75.1% | 75.1% | 226_227ins132 |
| 6 | human | 3574 | IL7 | interleukin 7 | XM_017013397.2 | 71.1% | 71.1% | 0_1ins153 |
| 7 | human | 3574 | IL7 | interleukin 7 | NM_001199888.1 | 64.9% | 64.9% | 227_228ins186 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccatgtttct tttaggtata tctttggact tcctcccctg atccttgttc 121 tgttgccagt agcatcatct gattgtgata ttgaaggtaa agatggcaaa caatatgaga 181 gtgttctaat ggtcagcatc gatcaattat tggacagcat gaaagaaatt ggtagcaatt 241 gcctgaataa tgaatttaac ttttttaaaa gacatatctg tgatgctaat aaggaaggta 301 tgtttttatt ccgtgctgct cgcaagttga ggcaatttct taaaatgaat agcactggtg 361 attttgatct ccacttatta aaagtttcag aaggcacaac aatactgttg aactgcactg 421 gccaggttaa aggaagaaaa ccagctgccc tgggtgaagc ccaaccaaca aagagtttgg 481 aagaaaataa atctttaaag gaacagaaaa aactgaatga cttgtgtttc ctaaagagac 541 tattacaaga gataaaaact tgttggaata aaattttgat gggcactaaa gaacactgcc 601 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 661 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 721 tta