Construct: ORF ccsbBroad304_00960

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008095.1_s304c1, BRDN0000403594
Derived from:
ccsbBroadEn_00960
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMAD1 (4086)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00960
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4086 SMAD1 SMAD family member 1 NM_001003688.1 100% 100%
2 human 4086 SMAD1 SMAD family member 1 NM_001354811.1 100% 100%
3 human 4086 SMAD1 SMAD family member 1 NM_001354812.1 100% 100%
4 human 4086 SMAD1 SMAD family member 1 NM_001354813.1 100% 100%
5 human 4086 SMAD1 SMAD family member 1 NM_001354814.1 100% 100%
6 human 4086 SMAD1 SMAD family member 1 NM_001354816.1 100% 100%
7 human 4086 SMAD1 SMAD family member 1 NM_001354817.1 100% 100%
8 human 4086 SMAD1 SMAD family member 1 NM_005900.3 100% 100%
9 human 4086 SMAD1 SMAD family member 1 XM_011531962.2 100% 100%
10 human 4086 SMAD1 SMAD family member 1 XM_011531964.2 100% 100%
11 mouse 17125 Smad1 SMAD family member 1 NM_008539.3 88.9% 99.1% (many diffs)
12 mouse 17125 Smad1 SMAD family member 1 XM_006530746.3 88.9% 99.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1461
ORF length:
1395
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tgtgacaagt ttattttcct ttacaagtcc agctgtgaag agacttcttg
121ggtggaaaca gggcgatgaa gaagaaaaat gggcagagaa agctgttgat gctttggtga
181aaaaactgaa gaaaaagaaa ggtgccatgg aggaactgga aaaggccttg agctgcccag
241ggcaaccgag taactgtgtc accattcccc gctctctgga tggcaggctg caagtctccc
301accggaaggg actgcctcat gtcatttact gccgtgtgtg gcgctggccc gatcttcaga
361gccaccatga actaaaacca ctggaatgct gtgagtttcc ttttggttcc aagcagaagg
421aggtctgcat caatccctac cactataaga gagtagaaag ccctgtactt cctcctgtgc
481tggttccaag acacagcgaa tataatcctc agcacagcct cttagctcag ttccgtaact
541taggacaaaa tgagcctcac atgccactca acgccacttt tccagattct ttccagcaac
601ccaacagcca cccgtttcct cactctccca atagcagtta cccaaactct cctgggagca
661gcagcagcac ctaccctcac tctcccacca gctcagaccc aggaagccct ttccagatgc
721cagctgatac gcccccacct gcttacctgc ctcctgaaga ccccatgacc caggatggct
781ctcagccgat ggacacaaac atgatggcgc ctcccctgcc ctcagaaatc aacagaggag
841atgttcaggc ggttgcttat gaggaaccaa aacactggtg ctctattgtc tactatgagc
901tcaacaatcg tgtgggtgaa gcgttccatg cctcctccac aagtgtgttg gtggatggtt
961tcactgatcc ttccaacaat aagaaccgtt tctgccttgg gctgctctcc aatgttaacc
1021ggaattccac tattgaaaac accaggcggc atattggaaa aggagttcat ctttattatg
1081ttggagggga ggtgtatgcc gaatgcctta gtgacagtag catctttgtg caaagtcgga
1141actgcaacta ccatcatgga tttcatccta ctactgtttg caagatccct agtgggtgta
1201gtctgaaaat ttttaacaac caagaatttg ctcagttatt ggcacagtct gtgaaccatg
1261gatttgagac agtctatgag cttacaaaaa tgtgtactat acgtatgagc tttgtgaagg
1321gctggggagc agaataccac cgccaggatg ttactagcac cccctgctgg attgagatac
1381atctgcacgg ccccctccag tggctggata aagttcttac tcaaatgggt tcacctcata
1441atcctatttc atctgtatct tacccaactt tcttgtacaa agtggttggt aagcctatcc
1501ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1561ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)