Construct: ORF ccsbBroad304_00996

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016992.1_s304c1, BRDN0000412190
Derived from:
ccsbBroadEn_00996
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BORCS8-MEF2B (4207)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00996
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100271849 MEF2B myocyte enhancer factor 2B NM_001367282.1 100% 100%
2 human 4207 BORCS8-MEF2B BORCS8-MEF2B readthrough NM_005919.4 100% 100%
3 human 100271849 MEF2B myocyte enhancer factor 2B NM_001145785.2 82.1% 68.8% 765_876del;1104_1105ins103
4 human 4207 BORCS8-MEF2B BORCS8-MEF2B readthrough NR_027308.2 64.7% 1_466del;1562_1691del
5 human 4207 BORCS8-MEF2B BORCS8-MEF2B readthrough NR_027307.2 60.7% 1_466del;1231_1342del;1674_1803del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1164
ORF length:
1095
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggggaggaaa aaaatccaga tctcccgcat cctggaccaa aggaatcggc
121aggtgacgtt caccaagcgg aagttcgggc tgatgaagaa ggcctatgag ctgagcgtgc
181tctgtgactg tgagatagcc ctcatcatct tcaacagcgc caaccgcctc ttccagtatg
241ccagcacgga catggaccgt gtgctgctga agtacacaga gtacagcgag ccccacgaga
301gccgcaccaa cactgacatc ctcgagacgc tgaagcggag gggcattggc ctcgatgggc
361cagagctgga gccggatgaa gggcctgagg agccaggaga gaagtttcgg aggctggcag
421gcgaaggggg tgatccggcc ttgccccgac cccggctgta tcctgcagct cctgctatgc
481ccagcccaga tgtggtatac ggggccttac cgccaccagg ctgtgacccc agtgggcttg
541gggaagcact gcccgcccag agccgcccat ctcccttccg accagcagcc cccaaagccg
601ggcccccagg ccTGGTGCAC CCTCTCTTCT CACCAAGCCA CCTCACCAGC AAGACACCAC
661CCCCACTGTA CCTGCCGACG GAAGGGCGGA GGTCAGACCT GCCTGGTGGC CTGGCTGGGC
721CCCGAGGGGG ACTAAACACC TCCAGAAGCC TCTACAGTGG CCTGCAGAAC CCCTGCTCCA
781CTGCAACTCC CGGACCCCCA CTGGGGAGCT TCCCCTTCCT CCCCGGAGGC CCCCCAGTGG
841GGGCCGAAGC CTGGGCGAGG AGGGTCCCCC AACCCGCGGC GCCTCCCCGC CGACCCCCCC
901AGTCAGCATC AAGTCTGAGC GCCTCTCTCC GGCCCCCGGG GGCCCCGGCG ACTTTCCTAA
961GACCTTCCCC TATCCCTTGC TCCTCGCCCG GTCCCTGGCA GAGCCTCTGC GGCCTGGGCC
1021CGCCCTGCGC CGGCTGCCCT TGGCCGACGG CTGGCCCCGG TAGGAGATCA CCCGGTGGCA
1081CCAGCCCAGA GCGCTCGCCA GGTACGGCGA GGGCACGTGG GGACCCCACC TCCCTCCAGG
1141CCTCTTCAGA GAAGACCCAA CAGTTGCCAA CTTTCTTGTA Caaagtggtt ggtaagccta
1201tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1261gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)