Transcript: Human NM_005919.4

Homo sapiens BORCS8-MEF2B readthrough (BORCS8-MEF2B), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
BORCS8-MEF2B (4207)
Length:
1513
CDS:
289..1386

Additional Resources:

NCBI RefSeq record:
NM_005919.4
NBCI Gene record:
BORCS8-MEF2B (4207)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005919.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232094 TATCCTGCAGCTCCTGCTATG pLKO_005 679 CDS 100% 6.000 3.000 Y BORCS8-MEF2B n/a
2 TRCN0000015739 CGCATCCTGGACCAAAGGAAT pLKO.1 316 CDS 100% 4.950 2.475 Y BORCS8-MEF2B n/a
3 TRCN0000232093 GTGAGATAGCCCTCATCATCT pLKO_005 410 CDS 100% 4.950 2.475 Y BORCS8-MEF2B n/a
4 TRCN0000232095 GGACTAAACACCTCCAGAAGC pLKO_005 949 CDS 100% 4.050 2.025 Y BORCS8-MEF2B n/a
5 TRCN0000232097 GGCGACTTTCCTAAGACCTTC pLKO_005 1167 CDS 100% 4.050 2.025 Y BORCS8-MEF2B n/a
6 TRCN0000232096 CCCAGTCAGCATCAAGTCTGA pLKO_005 1118 CDS 100% 2.640 1.320 Y BORCS8-MEF2B n/a
7 TRCN0000015738 CGGCGACTTTCCTAAGACCTT pLKO.1 1166 CDS 100% 2.640 1.320 Y BORCS8-MEF2B n/a
8 TRCN0000015740 GCCTCTTCAGAGAAGACCCAA pLKO.1 1360 CDS 100% 2.640 1.320 Y BORCS8-MEF2B n/a
9 TRCN0000015742 GCCAACCGCCTCTTCCAGTAT pLKO.1 439 CDS 100% 1.650 0.825 Y BORCS8-MEF2B n/a
10 TRCN0000015741 CCACTGTACCTGCCGACGGAA pLKO.1 883 CDS 100% 0.000 0.000 Y BORCS8-MEF2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005919.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00996 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00996 pLX_304 50.2% 100% 100% V5 n/a
3 TRCN0000491395 GATGTTCCGCCACACGCTGCTAAG pLX_317 22% 100% 100% V5 n/a
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