Transcript: Human NR_027307.2

Homo sapiens BORCS8-MEF2B readthrough (BORCS8-MEF2B), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
BORCS8-MEF2B (4207)
Length:
1803
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_027307.2
NBCI Gene record:
BORCS8-MEF2B (4207)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_027307.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232094 TATCCTGCAGCTCCTGCTATG pLKO_005 857 3UTR 100% 6.000 3.000 Y BORCS8-MEF2B n/a
2 TRCN0000015739 CGCATCCTGGACCAAAGGAAT pLKO.1 494 3UTR 100% 4.950 2.475 Y BORCS8-MEF2B n/a
3 TRCN0000232093 GTGAGATAGCCCTCATCATCT pLKO_005 588 3UTR 100% 4.950 2.475 Y BORCS8-MEF2B n/a
4 TRCN0000232095 GGACTAAACACCTCCAGAAGC pLKO_005 1127 3UTR 100% 4.050 2.025 Y BORCS8-MEF2B n/a
5 TRCN0000232097 GGCGACTTTCCTAAGACCTTC pLKO_005 1457 3UTR 100% 4.050 2.025 Y BORCS8-MEF2B n/a
6 TRCN0000232096 CCCAGTCAGCATCAAGTCTGA pLKO_005 1408 3UTR 100% 2.640 1.320 Y BORCS8-MEF2B n/a
7 TRCN0000015738 CGGCGACTTTCCTAAGACCTT pLKO.1 1456 3UTR 100% 2.640 1.320 Y BORCS8-MEF2B n/a
8 TRCN0000015740 GCCTCTTCAGAGAAGACCCAA pLKO.1 1650 3UTR 100% 2.640 1.320 Y BORCS8-MEF2B n/a
9 TRCN0000015742 GCCAACCGCCTCTTCCAGTAT pLKO.1 617 3UTR 100% 1.650 0.825 Y BORCS8-MEF2B n/a
10 TRCN0000015741 CCACTGTACCTGCCGACGGAA pLKO.1 1061 3UTR 100% 0.000 0.000 Y BORCS8-MEF2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_027307.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00996 pDONR223 100% 60.7% None 1_466del;1231_1342del;1674_1803del n/a
2 ccsbBroad304_00996 pLX_304 50.2% 60.7% V5 1_466del;1231_1342del;1674_1803del n/a
3 TRCN0000491395 GATGTTCCGCCACACGCTGCTAAG pLX_317 22% 60.7% V5 1_466del;1231_1342del;1674_1803del n/a
4 ccsbBroadEn_10202 pDONR223 100% 19.7% None 1_35del;116G>A;393_1803del n/a
5 ccsbBroad304_10202 pLX_304 0% 19.7% V5 1_35del;116G>A;393_1803del n/a
6 TRCN0000491305 GCGGGACACACGCACGAGTTCCTT pLX_317 90.1% 19.7% V5 1_35del;116G>A;393_1803del n/a
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