Construct: ORF ccsbBroad304_01001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013994.1_s304c1, BRDN0000399619
- Derived from:
- ccsbBroadEn_01001
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEST (4232)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01001
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4232 | MEST | mesoderm specific transcript | NM_002402.4 | 100% | 100% | |
| 2 | human | 4232 | MEST | mesoderm specific transcript | NM_177524.2 | 97.3% | 97.3% | 0_1ins27 |
| 3 | human | 4232 | MEST | mesoderm specific transcript | NM_177525.2 | 97.3% | 97.3% | 0_1ins27 |
| 4 | human | 4232 | MEST | mesoderm specific transcript | XM_017012218.2 | 97.3% | 97.3% | 0_1ins27 |
| 5 | human | 4232 | MEST | mesoderm specific transcript | XM_024446764.1 | 97.3% | 97.3% | 0_1ins27 |
| 6 | human | 4232 | MEST | mesoderm specific transcript | NM_001253900.1 | 95.8% | 95.8% | 137_138ins42 |
| 7 | human | 4232 | MEST | mesoderm specific transcript | XM_011516222.2 | 89.8% | 89.8% | 646_647ins102 |
| 8 | human | 4232 | MEST | mesoderm specific transcript | NM_001253901.1 | 87.1% | 87.1% | 0_1ins27;619_620ins102 |
| 9 | human | 4232 | MEST | mesoderm specific transcript | NM_001253902.1 | 87.1% | 87.1% | 0_1ins27;619_620ins102 |
| 10 | human | 4232 | MEST | mesoderm specific transcript | XM_024446765.1 | 87.1% | 87.1% | 0_1ins27;619_620ins102 |
| 11 | mouse | 17294 | Mest | mesoderm specific transcript | NM_008590.2 | 90.8% | 97.9% | (many diffs) |
| 12 | mouse | 17294 | Mest | mesoderm specific transcript | NM_001252292.1 | 88.6% | 92.4% | (many diffs) |
| 13 | mouse | 17294 | Mest | mesoderm specific transcript | NM_001252293.1 | 88.3% | 95.2% | (many diffs) |
| 14 | mouse | 17294 | Mest | mesoderm specific transcript | XM_017321427.1 | 78.5% | 81.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gcgccgagat cgcctccgca ggatgaggga gtggtgggtc caggtggggc 121 tgctggccgt gcccctgctt gctgcgtacc tgcacatccc accccctcag ctctcccctg 181 cccttcactc atggaagtct tcaggcaagt ttttcactta caagggactg cgtatcttct 241 accaagactc tgtgggtgtg gttggaagtc cagagatagt tgtgctttta cacggttttc 301 caacatccag ctacgactgg tacaagattt gggaaggtct gaccttgagg tttcatcggg 361 tgattgccct tgatttctta ggctttggct tcagtgacaa accgagacca catcactatt 421 ccatatttga gcaggccagc atcgtggaag cgcttttgcg gcatctgggg ctccagaacc 481 gcaggatcaa ccttctttct catgactatg gagatattgt tgctcaggag cttctctaca 541 ggtacaagca gaatcgatct ggtcggctta ccataaagag tctctgtctg tcaaatggag 601 gtatctttcc tgagactcac cgtccactcc ttctccaaaa gctactcaaa gatggaggtg 661 tgctgtcacc catcctcaca cgactgatga acttctttgt attctctcga ggtctcaccc 721 cagtctttgg gccgtatact cggccctctg agagtgagct gtgggacatg tgggcaggga 781 tccgcaacaa tgacgggaac ttagtcattg acagtctctt acagtacatc aatcagagga 841 agaagttcag aaggcgctgg gtgggagctc ttgcctctgt aactatcccc attcatttta 901 tctatgggcc attggatcct gtaaatccct atccagagtt tttggagctg tacaggaaaa 961 cgctgccgcg gtccacagtg tcgattctgg atgaccacat tagccactat ccacagctag 1021 aggatcccat gggcttcttg aatgcatata tgggcttcat caactccttc tgcccaactt 1081 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1141 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta