Construct: ORF ccsbBroad304_01001

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013994.1_s304c1, BRDN0000399619
Derived from:
ccsbBroadEn_01001
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MEST (4232)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01001
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4232 MEST mesoderm specific transcript NM_002402.4 100% 100%
2 human 4232 MEST mesoderm specific transcript NM_177524.2 97.3% 97.3% 0_1ins27
3 human 4232 MEST mesoderm specific transcript NM_177525.2 97.3% 97.3% 0_1ins27
4 human 4232 MEST mesoderm specific transcript XM_017012218.2 97.3% 97.3% 0_1ins27
5 human 4232 MEST mesoderm specific transcript XM_024446764.1 97.3% 97.3% 0_1ins27
6 human 4232 MEST mesoderm specific transcript NM_001253900.1 95.8% 95.8% 137_138ins42
7 human 4232 MEST mesoderm specific transcript XM_011516222.2 89.8% 89.8% 646_647ins102
8 human 4232 MEST mesoderm specific transcript NM_001253901.1 87.1% 87.1% 0_1ins27;619_620ins102
9 human 4232 MEST mesoderm specific transcript NM_001253902.1 87.1% 87.1% 0_1ins27;619_620ins102
10 human 4232 MEST mesoderm specific transcript XM_024446765.1 87.1% 87.1% 0_1ins27;619_620ins102
11 mouse 17294 Mest mesoderm specific transcript NM_008590.2 90.8% 97.9% (many diffs)
12 mouse 17294 Mest mesoderm specific transcript NM_001252292.1 88.6% 92.4% (many diffs)
13 mouse 17294 Mest mesoderm specific transcript NM_001252293.1 88.3% 95.2% (many diffs)
14 mouse 17294 Mest mesoderm specific transcript XM_017321427.1 78.5% 81.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1071
ORF length:
1005
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gcgccgagat cgcctccgca ggatgaggga gtggtgggtc caggtggggc
121tgctggccgt gcccctgctt gctgcgtacc tgcacatccc accccctcag ctctcccctg
181cccttcactc atggaagtct tcaggcaagt ttttcactta caagggactg cgtatcttct
241accaagactc tgtgggtgtg gttggaagtc cagagatagt tgtgctttta cacggttttc
301caacatccag ctacgactgg tacaagattt gggaaggtct gaccttgagg tttcatcggg
361tgattgccct tgatttctta ggctttggct tcagtgacaa accgagacca catcactatt
421ccatatttga gcaggccagc atcgtggaag cgcttttgcg gcatctgggg ctccagaacc
481gcaggatcaa ccttctttct catgactatg gagatattgt tgctcaggag cttctctaca
541ggtacaagca gaatcgatct ggtcggctta ccataaagag tctctgtctg tcaaatggag
601gtatctttcc tgagactcac cgtccactcc ttctccaaaa gctactcaaa gatggaggtg
661tgctgtcacc catcctcaca cgactgatga acttctttgt attctctcga ggtctcaccc
721cagtctttgg gccgtatact cggccctctg agagtgagct gtgggacatg tgggcaggga
781tccgcaacaa tgacgggaac ttagtcattg acagtctctt acagtacatc aatcagagga
841agaagttcag aaggcgctgg gtgggagctc ttgcctctgt aactatcccc attcatttta
901tctatgggcc attggatcct gtaaatccct atccagagtt tttggagctg tacaggaaaa
961cgctgccgcg gtccacagtg tcgattctgg atgaccacat tagccactat ccacagctag
1021aggatcccat gggcttcttg aatgcatata tgggcttcat caactccttc tgcccaactt
1081tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
1141agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)

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