Transcript: Mouse NM_001252292.1

Mus musculus mesoderm specific transcript (Mest), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Mest (17294)
Length:
2680
CDS:
341..1369

Additional Resources:

NCBI RefSeq record:
NM_001252292.1
NBCI Gene record:
Mest (17294)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001252292.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000032677 CCACATCAGTACTCCATATTT pLKO.1 704 CDS 100% 15.000 10.500 N Mest n/a
2 TRCN0000032676 CTCCAGTCTTTGGACCGTATA pLKO.1 1014 CDS 100% 10.800 7.560 N Mest n/a
3 TRCN0000032678 GAATGCATATATGGGCTTCAT pLKO.1 1336 CDS 100% 4.950 3.465 N Mest n/a
4 TRCN0000032674 GCCCTGAGATAGTTGTGCTTT pLKO.1 564 CDS 100% 4.950 3.465 N Mest n/a
5 TRCN0000032675 GCCCTTGATTTCTTAGGCTTT pLKO.1 662 CDS 100% 4.050 2.835 N Mest n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001252292.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01001 pDONR223 100% 88.6% 92.4% None (many diffs) n/a
2 ccsbBroad304_01001 pLX_304 0% 88.6% 92.4% V5 (many diffs) n/a
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