Construct: ORF ccsbBroad304_01009

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011342.1_s304c1, BRDN0000400382
Derived from:
ccsbBroadEn_01009
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MGST1 (4257)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01009
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4257 MGST1 microsomal glutathione S-tr... NM_001260511.1 100% 100%
2 human 4257 MGST1 microsomal glutathione S-tr... NM_020300.5 100% 100%
3 human 4257 MGST1 microsomal glutathione S-tr... NM_145764.2 100% 100%
4 human 4257 MGST1 microsomal glutathione S-tr... NM_145791.2 100% 100%
5 human 4257 MGST1 microsomal glutathione S-tr... NM_145792.2 100% 100%
6 human 4257 MGST1 microsomal glutathione S-tr... NM_001260512.1 61.1% 46.7% (many diffs)
7 human 4257 MGST1 microsomal glutathione S-tr... XM_006719079.1 59.8% 46.2% (many diffs)
8 human 4257 MGST1 microsomal glutathione S-tr... NM_001267598.2 53.9% 50.9% (many diffs)
9 human 4257 MGST1 microsomal glutathione S-tr... NR_048545.1 39.1% 1_90del;214_215ins95;461_849del
10 human 4257 MGST1 microsomal glutathione S-tr... NR_048547.1 37.1% 1_143del;267_268ins95;514_902del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
531
ORF length:
465
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt tgacctcacc caggtaatgg atgatgaagt attcatggct tttgcatcct
121atgcaacaat tattctttca aaaatgatgc ttatgagtac tgcaactgca ttctatagat
181tgacaagaaa ggtttttgcc aatccagaag actgtgtagc atttggcaaa ggagaaaatg
241ccaagaagta tcttcgaaca gatgacagag tagaacgtgt acgcagagcc cacctgaatg
301accttgaaaa tattattcca tttcttggaa ttggcctcct gtattccttg agtggtcccg
361acccctctac agccatcctg cacttcagac tatttgtcgg agcacggatc taccacacca
421ttgcatattt gacacccctt ccccagccaa atagagcttt gagttttttt gttggatatg
481gagttactct ttccatggct tacaggttgc tgaaaagtaa attgtacctg tacccaactt
541tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
601agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)