Construct: ORF ccsbBroad304_01019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011885.1_s304c1, BRDN0000409034
- Derived from:
- ccsbBroadEn_01019
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLH1 (4292)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01019
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4292 | MLH1 | mutL homolog 1 | NM_000249.3 | 100% | 100% | |
| 2 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354628.1 | 95.8% | 95.8% | 1892_1893ins93 |
| 3 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354629.1 | 95.6% | 95.6% | 206_207ins99 |
| 4 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354630.1 | 92.7% | 92.7% | 1730_1731ins165 |
| 5 | human | 4292 | MLH1 | mutL homolog 1 | NM_001258271.1 | 90.8% | 90.8% | 1894_1895ins207 |
| 6 | human | 4292 | MLH1 | mutL homolog 1 | XM_005265161.2 | 90.8% | 90.8% | 676_677ins207 |
| 7 | human | 4292 | MLH1 | mutL homolog 1 | NM_001167617.2 | 87% | 86.6% | 0_1ins289;9_10insGAGGT |
| 8 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354620.1 | 87% | 86.6% | 0_1ins289;9_10insGAGGT |
| 9 | human | 4292 | MLH1 | mutL homolog 1 | NM_001167618.2 | 68.1% | 68.1% | 0_1ins723 |
| 10 | human | 4292 | MLH1 | mutL homolog 1 | NM_001167619.2 | 68.1% | 68.1% | 0_1ins723 |
| 11 | human | 4292 | MLH1 | mutL homolog 1 | NM_001258273.1 | 68.1% | 68.1% | 0_1ins723 |
| 12 | human | 4292 | MLH1 | mutL homolog 1 | NM_001258274.2 | 68.1% | 68.1% | 0_1ins723 |
| 13 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354615.1 | 68.1% | 68.1% | 0_1ins723 |
| 14 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354616.1 | 68.1% | 68.1% | 0_1ins723 |
| 15 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354617.1 | 68.1% | 68.1% | 0_1ins723 |
| 16 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354618.1 | 68.1% | 68.1% | 0_1ins723 |
| 17 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354619.1 | 68.1% | 68.1% | 0_1ins723 |
| 18 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354621.1 | 54.8% | 54.8% | 0_1ins1023 |
| 19 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354622.1 | 54.8% | 54.8% | 0_1ins1023 |
| 20 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354623.1 | 54.8% | 54.8% | 0_1ins1023 |
| 21 | human | 4292 | MLH1 | mutL homolog 1 | XM_017006450.2 | 54.8% | 54.8% | 0_1ins1023 |
| 22 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354624.1 | 52.6% | 52.6% | 0_1ins1074 |
| 23 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354625.1 | 52.6% | 52.6% | 0_1ins1074 |
| 24 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354626.1 | 52.6% | 52.6% | 0_1ins1074 |
| 25 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354627.1 | 52.6% | 52.6% | 0_1ins1074 |
| 26 | mouse | 17350 | Mlh1 | mutL homolog 1 | NM_026810.2 | 86.7% | 88.4% | (many diffs) |
| 27 | mouse | 17350 | Mlh1 | mutL homolog 1 | XM_011242935.2 | 79.9% | 79.7% | (many diffs) |
| 28 | mouse | 17350 | Mlh1 | mutL homolog 1 | XR_379848.2 | 65.1% | (many diffs) | |
| 29 | mouse | 17350 | Mlh1 | mutL homolog 1 | XR_001778805.1 | 64% | (many diffs) | |
| 30 | mouse | 17350 | Mlh1 | mutL homolog 1 | XR_379849.3 | 59.6% | (many diffs) | |
| 31 | mouse | 17350 | Mlh1 | mutL homolog 1 | XM_006511946.3 | 58.2% | 53.9% | (many diffs) |
| 32 | mouse | 17350 | Mlh1 | mutL homolog 1 | NM_001324522.1 | 58.1% | 58.2% | (many diffs) |
| 33 | mouse | 17350 | Mlh1 | mutL homolog 1 | XM_006511948.3 | 58.1% | 58.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2334
- ORF length:
- 2268
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gttcgtggca ggggttattc ggcggctgga cgagacagtg gtgaaccgca 121 tcgcggcggg ggaagttatc cagcggccag ctaatgctat caaagagatg attgagaact 181 gtttagatgc aaaatccaca agtattcaag tgattgttaa agagggaggc ctgaagttga 241 ttcagatcca agacaatggc accgggatca ggaaagaaga tctggatatt gtatgtgaaa 301 ggttcactac tagtaaactg cagtcctttg aggatttagc cagtatttct acctatggct 361 ttcgaggtga ggctttggcc agcataagcc atgtggctca tgttactatt acaacgaaaa 421 cagctgatgg aaagtgtgca tacagagcaa gttactcaga tggaaaactg aaagcccctc 481 ctaaaccatg tgctggcaat caagggaccc agatcacggt ggaggacctt ttttacaaca 541 tagccacgag gagaaaagct ttaaaaaatc caagtgaaga atatgggaaa attttggaag 601 ttgttggcag gtattcagta cacaatgcag gcattagttt ctcagttaaa aaacaaggag 661 agacagtagc tgatgttagg acactaccca atgcctcaac cgtggacaat attcgctcca 721 tctttggaaa tgctgttagt cgagaactga tagaaattgg atgtgaggat aaaaccctag 781 ccttcaaaat gaatggttac atatccaatg caaactactc agtgaagaag tgcatcttct 841 tactcttcat caaccatcgt ctggtagaat caacttcctt gagaaaagcc atagaaacag 901 tgtatgcagc ctatttgccc aaaaacacac acccattcct gtacctcagt ttagaaatca 961 gtccccagaa tgtggatgtt aatgtgcacc ccacaaagca tgaagttcac ttcctgcacg 1021 aggagagcat cctggagcgg gtgcagcagc acatcgagag caagctcctg ggctccaatt 1081 cctccaggat gtacttcacc cagactttgc taccaggact tgctggcccc tctggggaga 1141 tggttaaatc cacaacaagt ctgacctcgt cttctacttc tggaagtagt gataaggtct 1201 atgcccacca gatggttcgt acagattccc gggaacagaa gcttgatgca tttctgcagc 1261 ctctgagcaa acccctgtcc agtcagcccc aggccattgt cacagaggat aagacagata 1321 tttctagtgg cagggctagg cagcaagatg aggagatgct tgaactccca gcccctgctg 1381 aagtggctgc caaaaatcag agcttggagg gggatacaac aaaggggact tcagaaatgt 1441 cagagaagag aggacctact tccagcaacc ccagaaagag acatcgggaa gattctgatg 1501 tggaaatggt ggaagatgat tcccgaaagg aaatgactgc agcttgtacc ccccggagaa 1561 ggatcattaa cctcactagt gttttgagtc tccaggaaga aattaatgag cagggacatg 1621 aggttctccg ggagatgttg cataaccact ccttcgtggg ctgtgtgaat cctcagtggg 1681 ccttggcaca gcatcaaacc aagttatacc ttctcaacac caccaagctt agtgaagaac 1741 tgttctacca gatactcatt tatgattttg ccaattttgg tgttctcagg ttatcggagc 1801 cagcaccgct ctttgacctt gccatgcttg ccttagatag tccagagagt ggctggacag 1861 aggaagatgg tcccaaagaa ggacttgctg aatacattgt tgagtttctg aagaagaagg 1921 ctgagatgct tgcagactat ttctctttgg aaattgatga ggaagggaac ctgattggat 1981 taccccttct gattgacaac tatgtgcccc ctttggaggg actgcctatc ttcattcttc 2041 gactagccac tgaggtgaat tgggacgaag aaaaggaatg ttttgaaagc ctcagtaaag 2101 aatgcgctat gttctattcc atccggaagc agtacatatc tgaggagtcg accctctcag 2161 gccagcagag tgaagtgcct ggctccattc caaactcctg gaagtggact gtggaacaca 2221 ttgtctataa agccttgcgc tcacacattc tgcctcctaa acatttcaca gaagatggaa 2281 atatcctgca gcttgctaac ctgcctgatc tatacaaagt ctttgagagg tgttacccaa 2341 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2401 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta