Construct: ORF ccsbBroad304_01035

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008013.1_s304c1, BRDN0000410420
Derived from:
ccsbBroadEn_01035
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MSH5 (4439)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01035
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4439 MSH5 mutS homolog 5 NM_172165.3 100% 100%
2 human 4439 MSH5 mutS homolog 5 NM_002441.4 99.8% 99.8% 1959_1960insCAG
3 human 4439 MSH5 mutS homolog 5 NM_172166.4 99.8% 99.8% 1959_1960insCAG
4 human 4439 MSH5 mutS homolog 5 NM_025259.5 94.4% 94.4% 538_588del;2281_2282ins90
5 human 100532732 MSH5-SAPCD1 MSH5-SAPCD1 readthrough (NM... NR_037846.1 61.4% (many diffs)
6 mouse 17687 Msh5 mutS homolog 5 NM_001146215.2 86.4% 88.4% (many diffs)
7 mouse 17687 Msh5 mutS homolog 5 NM_013600.3 86.4% 88.4% (many diffs)
8 mouse 17687 Msh5 mutS homolog 5 XM_011246314.1 86.3% 88.3% (many diffs)
9 mouse 17687 Msh5 mutS homolog 5 XM_006523771.3 85.1% 86.3% (many diffs)
10 mouse 17687 Msh5 mutS homolog 5 XM_006523770.1 84.6% 85.6% (many diffs)
11 mouse 17687 Msh5 mutS homolog 5 XM_006523778.1 81.3% 83.1% (many diffs)
12 mouse 17687 Msh5 mutS homolog 5 XM_017317278.1 78% 80.2% (many diffs)
13 mouse 17687 Msh5 mutS homolog 5 XM_017317279.1 77.9% 80% (many diffs)
14 mouse 17687 Msh5 mutS homolog 5 XM_017317280.1 76.7% 78.8% (many diffs)
15 mouse 17687 Msh5 mutS homolog 5 XM_017317281.1 75.8% 78.1% (many diffs)
16 mouse 17687 Msh5 mutS homolog 5 XM_017317282.1 75.7% 77.7% (many diffs)
17 mouse 17687 Msh5 mutS homolog 5 NR_130640.1 74.5% (many diffs)
18 mouse 17687 Msh5 mutS homolog 5 XM_017317283.1 74.1% 75.4% (many diffs)
19 mouse 17687 Msh5 mutS homolog 5 XM_017317284.1 72.9% 74.3% (many diffs)
20 mouse 17687 Msh5 mutS homolog 5 XM_017317285.1 72% 73.7% (many diffs)
21 mouse 17687 Msh5 mutS homolog 5 NR_130641.1 71.9% (many diffs)
22 mouse 17687 Msh5 mutS homolog 5 XM_006523780.3 70.8% 73.6% (many diffs)
23 mouse 17687 Msh5 mutS homolog 5 XR_001782050.1 66% (many diffs)
24 mouse 17687 Msh5 mutS homolog 5 XR_001782053.1 64.6% (many diffs)
25 mouse 17687 Msh5 mutS homolog 5 XR_001782049.1 62.5% (many diffs)
26 mouse 17687 Msh5 mutS homolog 5 XR_001782051.1 57.3% (many diffs)
27 mouse 17687 Msh5 mutS homolog 5 XR_001782054.1 56.1% (many diffs)
28 mouse 17687 Msh5 mutS homolog 5 XM_017317286.1 55.7% 56.9% (many diffs)
29 mouse 17687 Msh5 mutS homolog 5 XR_001782052.1 52.1% (many diffs)
30 mouse 17687 Msh5 mutS homolog 5 XM_017317288.1 37.5% 36.1% (many diffs)
31 mouse 17687 Msh5 mutS homolog 5 XM_017317289.1 37.5% 36.1% (many diffs)
32 mouse 17687 Msh5 mutS homolog 5 XM_017317290.1 37.5% 36.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2571
ORF length:
2505
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ctccttagga gcgaacccaa ggaggacacc gcagggaccg agacctgggg
121cggcctcctc cggcttcccc agcccggccc cagtgccggg ccccagggag gccgaggagg
181aggaagtcga ggaggaggag gagctggccg agatccatct gtgtgtgctg tggaattcag
241gatacttggg cattgcctac tatgatacta gtgactccac tatccacttc atgccagatg
301ccccagacca cgagagcctc aagcttctcc agagagttct ggatgagatc aatccccagt
361ctgttgttac gagtgccaaa caggatgaga atatgactcg atttctggga aagcttgcct
421cccaggagca cagagagcct aaaagacctg aaatcatatt tttgccaagt gtggattttg
481gtctggagat aagcaaacaa cgcctccttt ctggaaacta ctccttcatc ccagacgcca
541tgactgccac tgagaaaatc ctcttcctct cttccattat tccctttgac tgcctcctca
601cagttcgagc acttggaggg ctgctgaagt tcctgggtcg aagaagaatc ggggttgaac
661tggaagacta taatgtcagc gtccccatcc tgggctttaa gaaatttatg ttgactcatc
721tggtgaacat agatcaagac acttacagtg ttctacagat ttttaagagt gagtctcacc
781cctcagtgta caaagtggcc agtggactga aggaggggct cagcctcttt ggaatcctca
841acagatgcca ctgtaagtgg ggagagaagc tgctcaggct atggttcaca cgtccgactc
901atgacctggg ggagctcagt tctcgtctgg acgtcattca gttttttctg ctgccccaga
961atctggacat ggctcagatg ctgcatcggc tcctgggtca catcaagaac gtgcctctga
1021ttctgaaacg catgaagttg tcccacacca aggtcagcga ctggcaggtt ctctacaaga
1081ctgtgtacag tgccctgggc ctgagggatg cctgccgctc cctgccgcag tccatccagc
1141tctttcggga cattgcccaa gagttctctg atgacctgca ccatatcgcc agcctcattg
1201ggaaagtagt ggactttgag ggcagccttg ctgaaaatcg cttcacagtc ctccccaaca
1261tagatcctga aattgatgag aaaaagcgaa gactgatggg acttcccagt ttccttactg
1321aggttgcccg caaggagctg gagaatctgg actcccgtat tccttcatgc agtgtcatct
1381acatccctct gattggcttc cttctttcta ttccccgcct gccttccatg gtagaggcca
1441gtgactttga gattaatgga ctggacttca tgtttctctc agaggagaag ctgcactatc
1501gtagtgcccg aaccaaggag ctggatgcat tgctggggga cctgcactgc gagatccggg
1561accaggagac gctgctgatg taccagctac agtgccaggt gctggcacga gcagctgtct
1621taacccgagt attggacctt gcctcccgcc tggacgtcct gctggctctt gccagtgctg
1681cccgggacta tggctactca aggccgcgtt actccccaca agtccttggg gtacgaatcc
1741agaatggcag acatcctctg atggaactct gtgcccgaac ctttgtgccc aactccacag
1801aatgtggtgg ggacaaaggg agggtcaaag tcatcactgg acccaactca tcagggaaga
1861gcatatacct caaacaggta ggcttgatca cattcatggc cctggtaggc agctttgtgc
1921cagcagagga ggccgaaatt ggggcagtag acgccatctt cacacgaatt catagctgcg
1981aatccatctc ccttggcctc tccaccttca tgatcgacct caaccagcag gtggcgaaag
2041cagtgaacaa tgccactgca cagtcgctgg tccttattga tgaatttgga aagggaacca
2101acacggtgga tgggctcgcg cttctggccg ctgtgctccg acactggctg gcacgtggac
2161ccacatgccc ccacatcttt gtggccacca actttctgag ccttgttcag ctacaactgc
2221tgccacaagg gcccctggtg cagtatttga ccatggagac ctgtgaggat ggcaacgatc
2281ttgtcttctt ctatcaggtt tgcgaaggtg ttgcgaaggc cagccatgcc tcccacacag
2341ctgcccaggc tgggcttcct gacaagcttg tggctcgtgg caaggaggtc tcagacttga
2401tccgcagtgg aaaacccatc aagcctgtca aggatttgct aaagaagaac caaatggaaa
2461attgccagac attagtggat aagtttatga aactggattt ggaagatcct aacctggact
2521tgaacgtttt catgagccag gaagtgctgc ctgctgccac cagcatcctc tgcccaactt
2581tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
2641agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

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