Construct: ORF ccsbBroad304_01050

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF009427.1_s304c1, BRDN0000408500
• Derived from: ccsbBroadEn_01050
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: MUC1 (4582)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_01050
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001018016.3	100%	100%
2	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001018017.3	96.5%	96.5%	54_55ins27
3	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204287.2	93.6%	93.6%	187_240del
4	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204291.1	91.2%	91.2%	186_187ins69
5	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204292.1	90.5%	85.9%	186_187insTTTAATTCCTCTCTGGAAG;235_236ins56
6	human	4582	MUC1	mucin 1, cell surface assoc...	NM_002456.6	90.4%	90.4%	54_55ins27;160_213del
7	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204289.2	90.1%	90.1%	186_187ins78
8	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204294.2	87.1%	82.5%	54_55ins27;159_160insTTTAATTCCTCTCTGGAAG;208_209ins56
9	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204288.2	82.9%	59.9%	447_448ins32;657_658ins103
10	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204290.2	82.1%	82.1%	55_56ins63;123_124ins78
11	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204296.2	80.3%	69.3%	186_187insTTTAATTCCTCTCTGGAAG;291_292ins137
12	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001044390.3	76.8%	65.9%	54_55ins27;159_160insTTTAATTCCTCTCTGGAAG;264_265ins137
13	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204295.1	71.5%	71.5%	254_255ins225
14	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204293.2	63.8%	48.9%	(many diffs)
15	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001044392.3	60.2%	60.2%	253_254ins315
16	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001044393.3	59.8%	25.8%	(many diffs)
17	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001044391.3	56.8%	56.8%	54_55ins27;226_227ins315
18	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204297.2	56.3%	56.3%	187_240del;307_308ins315
19	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204286.1	54.5%	54.5%	187_846del
20	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001204285.2	52.6%	52.6%	54_55ins27;160_819del
21	human	4582	MUC1	mucin 1, cell surface assoc...	NM_001371720.1	19.8%	17.7%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

69

ORF end:

861

ORF length:

792

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcaccat gacaccgggc acccagtctc ctttcttcct gctgctgctc ctcacagtgc
121	ttacagctac cacagcccct aaacccgcaa cagttgttac gggttctggt catgcaagct
181	ctaccccagg tggagaaaag gagacttcgg ctacccagag aagttcagtg cccagctcta
241	ctgagaagaa tgcttttaat tcctctctgg aagatcccag caccgactac taccaagagc
301	tgcagagaga catttctgaa atgtttttgc agatttataa acaagggggt tttctgggcc
361	tctccaatat taagttcagg ccaggatctg tggtggtaca attgactctg gccttccgag
421	aaggtaccat caatgtccac gacgtggaga cacagttcaa tcagtataaa acggaagcag
481	cctctcgata taacctgacg atctcagacg tcagcgtgag tgatgtgcca tttcctttct
541	ctgcccagtc tggggctggg gtgccaggct ggggcatcgc gctgctggtg ctggtctgtg
601	ttctggttgc gctggccatt gtctatctca ttgccttggc tgtctgtcag tgccgccgaa
661	agaactacgg gcagctggac atctttccag cccgggatac ctaccatcct atgagcgagt
721	accccaccta ccacacccat gggcgctatg tgccccctag cagtaccgat cgtagcccct
781	atgagaaggt ttctgcaggt aatggtggca gcagcctctc ttacacaaac ccagcagtgg
841	cagccacttc tgccaacttg ttgccaactt tcttgtacaa agtggttggt aagcctatcc
901	ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
961	ttaagtcgac aatcaacctc tggatta

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