Construct: ORF ccsbBroad304_01058
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014517.1_s304c1, BRDN0000462313
- Derived from:
- ccsbBroadEn_01058
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYO6 (4646)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01058
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4646 | MYO6 | myosin VI | NM_004999.4 | 100% | 100% | |
| 2 | human | 4646 | MYO6 | myosin VI | NM_001368865.1 | 99.3% | 99.2% | 3108_3134del |
| 3 | human | 4646 | MYO6 | myosin VI | XM_024446447.1 | 99.3% | 99.2% | 3108_3134del |
| 4 | human | 4646 | MYO6 | myosin VI | XM_005248722.4 | 99.2% | 99.2% | 3412_3413ins27 |
| 5 | human | 4646 | MYO6 | myosin VI | XM_005248724.4 | 98.9% | 98.9% | 3137_3138ins39 |
| 6 | human | 4646 | MYO6 | myosin VI | NM_001368866.1 | 98.6% | 98.4% | 3108_3134del;3439_3440ins27 |
| 7 | human | 4646 | MYO6 | myosin VI | XM_005248721.4 | 98.2% | 98.2% | 3108_3134del;3164_3165ins39 |
| 8 | human | 4646 | MYO6 | myosin VI | XM_017010899.2 | 98.2% | 98.2% | 3137_3138ins39;3373_3374ins27 |
| 9 | human | 4646 | MYO6 | myosin VI | NM_001300899.2 | 98.2% | 98.2% | 3105_3106ins69 |
| 10 | human | 4646 | MYO6 | myosin VI | NM_001368138.1 | 97.8% | 97.8% | 551_552insAGCTAACCCACTCCT;3090_3091ins69 |
| 11 | human | 4646 | MYO6 | myosin VI | NM_001368137.1 | 97.6% | 97.4% | 3108_3134del;3164_3165ins39;3400_3401ins27 |
| 12 | human | 4646 | MYO6 | myosin VI | NM_001368136.1 | 97.5% | 97.4% | 3105_3106ins69;3343_3344ins27 |
| 13 | human | 4646 | MYO6 | myosin VI | NR_160538.1 | 43.1% | (many diffs) | |
| 14 | human | 4646 | MYO6 | myosin VI | NM_001368139.1 | 21.6% | 21.3% | (many diffs) |
| 15 | human | 4646 | MYO6 | myosin VI | NM_001368140.1 | 21.6% | 21.3% | (many diffs) |
| 16 | mouse | 17920 | Myo6 | myosin VI | XM_006510835.3 | 87.9% | 95.8% | (many diffs) |
| 17 | mouse | 17920 | Myo6 | myosin VI | XM_011242667.1 | 87.7% | 95.6% | (many diffs) |
| 18 | mouse | 17920 | Myo6 | myosin VI | XM_006510836.2 | 87.2% | 95% | (many diffs) |
| 19 | mouse | 17920 | Myo6 | myosin VI | XM_011242668.2 | 87% | 94.8% | (many diffs) |
| 20 | mouse | 17920 | Myo6 | myosin VI | NM_001039546.2 | 86.9% | 95% | (many diffs) |
| 21 | mouse | 17920 | Myo6 | myosin VI | XM_011242669.1 | 86.7% | 94.6% | (many diffs) |
| 22 | mouse | 17920 | Myo6 | myosin VI | XM_011242670.2 | 86.6% | 94.8% | (many diffs) |
| 23 | mouse | 17920 | Myo6 | myosin VI | XM_006510838.1 | 86.1% | 94.3% | (many diffs) |
| 24 | mouse | 17920 | Myo6 | myosin VI | XM_017313191.1 | 85.9% | 94% | (many diffs) |
| 25 | mouse | 17920 | Myo6 | myosin VI | XM_011242671.1 | 45.3% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3924
- ORF length:
- 3855
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggatgga aagcccgttt gggcgccaca ccctacagat ggatttcaga 121 tgggcaatat tgtggatatt ggccccgaca gcttaacaat tgaacccttg aatcagaaag 181 gcaagacatt tttggctctc ataaaccaag tgtttcctgc agaagaggac agtaaaaaag 241 atgtggaaga taactgttca ctaatgtatt taaatgaagc cacactgctc cataatatca 301 aagttcgata tagtaaagac agaatttata catatgtcgc caacattctg attgcagtga 361 atccatactt tgacatacct aaaatatatt cttcagaagc aataaagtca tatcaaggaa 421 aatctcttgg gacaagacca cctcatgtct ttgcaattgc tgataaagct tttcgagaca 481 tgaaggtgct caagatgagt cagtctatca ttgtatctgg agaatcagga gccggcaaaa 541 cagaaaatac aaaatttgtt ctaagatacc tgactgaatc ctatggaaca ggtcaagata 601 ttgatgacag aattgttgaa gctaacccac tcctagaagc ctttggaaat gcgaagactg 661 ttcgcaacaa taatagcagt cgatttggga aatttgtaga aatacatttt aatgaaaaga 721 gctcagttgt tggaggattt gtttcacatt atctcctaga gaaatctagg atctgtgttc 781 aaggcaaaga ggaaagaaat tatcatatct tttataggtt gtgtgctggt gcttctgaag 841 atattagaga aaaacttcat ttgagttcac cagataattt tcggtattta aaccgaggct 901 gcactagata ctttgctaac aaagaaactg acaaacagat tttacagaac cgcaaaagtc 961 ctgagtacct taaggcaggt tctatgaaag atcctctgct agatgaccat ggtgatttta 1021 ttagaatgtg cacggctatg aaaaaaattg gtttggatga tgaagaaaag cttgatctct 1081 tccgggtagt agctggcgtc ctgcaccttg gaaatattga ttttgaggaa gctggcagca 1141 cttcaggtgg ttgtaatctg aagaataaat ctgctcagtc tttggaatat tgtgctgaat 1201 tactgggttt ggaccaagat gatcttcgag taagtttgac cacaagagtc atgctaacaa 1261 cagcaggggg caccaaagga acagttataa aggtacctct gaaagtggag caagcaaaca 1321 atgctcgtga tgccctggca aagacagtgt atagccatct ttttgatcat gtggtaaaca 1381 gagtaaatca gtgttttcct tttgaaacat catcctattt tattggagtc ctagatattg 1441 ctggttttga gtactttgag cataacagtt ttgaacaatt ttgcatcaac tattgcaatg 1501 aaaaacttca acaatttttt aatgaaagga ttctgaagga ggaacaagaa ctctatcaaa 1561 aagaaggttt aggtgttaat gaagtgcatt atgtggataa tcaggactgt atagatttaa 1621 ttgaagccaa attagtggga atactggata ttttggatga agaaaatcgc cttccccagc 1681 caagtgatca acactttaca tctgcagttc accaaaagca caaggatcat tttcgactca 1741 ctattcccag aaaatctaag ctggcagttc ataggaatat cagagacgac gaaggcttca 1801 ttatcaggca ttttgcgggg gcagtgtgct atgaaacaac ccagtttgtg gagaaaaata 1861 atgatgcttt acatatgtct cttgaatcct taatatgtga atccagagat aagtttatac 1921 gggaattatt tgaatcatcc acaaataaca acaaagatac taaacaaaaa gcaggaaaac 1981 ttagcttcat cagcgtggga aacaagttta agacacagtt aaatttgctt ctggataaac 2041 ttcgaagtac tggagcaagc tttattcgtt gcatcaaacc taacttaaag atgacaagcc 2101 accactttga aggtgctcaa attctgtctc agcttcagtg ttcagggatg gtgtctgttt 2161 tggacttgat gcagggtggt tacccatcac gagcttcatt tcatgaactc tacaacatgt 2221 acaaaaagta tatgccagat aaacttgcaa gattggatcc aagactattt tgtaaggctt 2281 tgtttaaagc tttgggctta aatgaaaatg actacaagtt tgggttaacc aaagtatttt 2341 ttagacctgg caagtttgca gaatttgatc agatcatgaa gtctgaccct gaccacttag 2401 cagagttggt taaaagagtc aatcactggc tcacatgcag tcgctggaag aaagttcagt 2461 ggtgctcact ctcagtcatc aaattgaaaa acaaaataaa atatcgagct gaagcctgca 2521 ttaaaatgca aaaaactatt cgaatgtggc tttgcaagag gagacacaaa cctcgcattg 2581 atggtctggt taaggtgggc acactgaaaa aacgacttga taaatttaat gaggtagtca 2641 gtgtgttgaa agatggaaaa cccgagatga ataaacagat caagaatctg gaaatttcta 2701 ttgatacttt gatggccaaa attaagtcca ctatgatgac gcaggaacaa atccagaaag 2761 aatatgatgc actggttaaa agctcagagg aactcctcag tgcattacag aaaaaaaaac 2821 agcaggaaga ggaagcagaa aggctgaggc gtattcaaga agaaatggaa aaggaaagaa 2881 aaagacgtga agaagacgaa aaacgtcgaa gaaaggaaga ggaggaaagg cggatgaaac 2941 ttgagatgga agcaaagaga aaacaagaag aagaagagag aaagaaaagg gaagatgatg 3001 aaaaacgcat tcaagctgaa gtggaggcac agctggcccg acagaaggag gaggaatccc 3061 aacagcaagc agttctggag caggagcgca gggaccggga gctggccctg aggattgccc 3121 agagtgaagc cgagctcatc agtgatgagg cccaggccga cctggcgctg cggagaaatg 3181 atggaacaag acccaaaatg acaccggaac aaatggccaa agaaatgtca gaatttttga 3241 gtagaggtcc tgctgtacta gccaccaaag cagctgctgg tactaagaaa tatgatctta 3301 gtaaatggaa atatgcagaa ctacgtgata ccatcaatac ttcttgtgat attgagctcc 3361 tggcagcttg cagagaagaa tttcatagga gactaaaagt gtatcatgct tggaaatcta 3421 agaacaagaa gagaaatact gaaacagagc aacgtgctcc aaagtctgtt actgattatg 3481 attttgcacc atttttgaac aattcacctc agcaaaaccc agcagctcag attcctgcca 3541 ggcagcggga gattgaaatg aaccgacagc aacgcttctt ccgcatccca ttcatccgcc 3601 ctgccgacca gtacaaagac cctcagagta agaaaaaagg ctggtggtat gcccattttg 3661 atggaccatg gattgcccgg caaatggaac tccatcctga caagccaccc atcctacttg 3721 tggctggtaa ggacgacatg gagatgtgtg agctgaatct tgaggagact ggcctgactc 3781 ggaagcgtgg tgctgagatc ttgccaagac agtttgaaga aatctgggaa cgctgtggag 3841 gcatccagta ccttcagaat gcgattgaga gcagacaggc tcggcccacc tatgcaacag 3901 ccatgctgca gagtctgtta aagttgccaa ctttcttgta caaagtggtt ggtaagccta 3961 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 4021 gcgttaagtc gacaatcaac ctctggatta