Construct: ORF ccsbBroad304_01063
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014906.1_s304c1, BRDN0000398777
- Derived from:
- ccsbBroadEn_01063
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDP (4693)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01063
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4693 | NDP | norrin cystine knot growth ... | NM_000266.4 | 100% | 100% | |
| 2 | mouse | 17986 | Ndp | Norrie disease (pseudogliom... | NM_010883.3 | 90.4% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag aaaacatgta ctagctgcat ccttttctat gctctccctg ctggtgataa 121 tgggagatac agacagtaaa acggacagct cattcataat ggactcggac cctcgacgct 181 gcatgaggca ccactatgtg gattctatca gtcacccatt gtacaagtgt agctcaaaga 241 tggtgctcct ggccaggtgc gaggggcact gcagccaggc gtcacgctcc gagcctttgg 301 tgtcgttcag cactgtcctc aagcaaccct tccgttcctc ctgtcactgc tgccggcccc 361 agacttccaa gctgaaggca ctgcggctgc gatgctcagg gggcatgcga ctcactgcca 421 cctaccggta catcctctcc tgtcactgcg aggaatgcaa ttcctgccca actttcttgt 481 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 541 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a