Construct: ORF ccsbBroad304_01088

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006847.1_s304c1, BRDN0000405001
Derived from:
ccsbBroadEn_01088
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NFATC3 (4775)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01088
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4775 NFATC3 nuclear factor of activated... NM_173165.3 100% 100%
2 human 4775 NFATC3 nuclear factor of activated... NM_004555.4 97.5% 96.4% (many diffs)
3 human 4775 NFATC3 nuclear factor of activated... NM_173163.3 97.5% 96.5% (many diffs)
4 mouse 18021 Nfatc3 nuclear factor of activated... NM_010901.2 88.8% 88.9% (many diffs)
5 mouse 18021 Nfatc3 nuclear factor of activated... XM_006530753.2 87.7% 86.5% (many diffs)
6 mouse 18021 Nfatc3 nuclear factor of activated... XM_006530754.3 86.6% 86.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3291
ORF length:
3225
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac tactgcaaac tgtggcgccc acgacgagct cgacttcaaa ctcgtctttg
121gcgaggacgg ggcgccggcg ccgccgcccc cgggctcgcg gcctgcagat cttgagccag
181atgattgtgc atccatttac atctttaatg tagatccacc tccatctact ttaaccacac
241cactttgctt accacatcat ggattaccgt ctcactcttc tgttttgtca ccatcgtttc
301agctccaaag tcacaaaaac tatgaaggaa cttgtgagat tcctgaatct aaatatagcc
361cattaggtgg tcccaaaccc tttgagtgcc caagtattca aattacatct atctctccta
421actgtcatca agaattagat gcacatgaag atgacctaca gataaatgac ccagaacggg
481aatttttgga aaggccttct agagatcatc tctatcttcc tcttgagcca tcctaccggg
541agtcttctct tagtcctagt cctgccagca gcatctcttc taggagttgg ttctctgatg
601catcttcttg tgaatcgctt tcacatattt atgatgatgt ggactcagag ttgaatgaag
661ctgcagcccg atttaccctt ggatcccctc tgacttctcc tggtggctct ccagggggct
721gccctggaga agaaacttgg catcaacagt atggacttgg acactcatta tcacccaggc
781aatctccttg ccactctcct agatccagtg tcactgatga gaattggctg agccccaggc
841cagcctcagg accctcatca aggcccacat ccccctgtgg gaaacggagg cactccagtg
901ctgaagtttg ttatgctggg tccctttcac cccatcactc acctgttcct tcacctggtc
961actcccccag gggaagtgtg acagaagata cgtggctcaa tgcttctgtc catggtgggt
1021caggccttgg ccctgcagtt tttccatttc agtactgtgt agagactgac atccctctca
1081aaacaaggaa aacttctgaa gatcaagctg ccatactacc aggaaaatta gagctgtgtt
1141cagatgacca agggagttta tcaccagccc gggagacttc aatagatgat ggccttggat
1201ctcagtatcc tttaaagaaa gattcatgtg gtgatcagtt tctttcagtt ccttcaccct
1261ttacctggag caaaccaaag cctggccaca cccctatatt tcgcacatct tcattacctc
1321cactagactg gcctttacca gctcattttg gacaatgtga actgaaaata gaagtgcaac
1381ctaaaactca tcatcgagcc cattatgaaa ctgaaggtag ccgaggggca gtaaaagcat
1441ctactggggg acatcctgtt gtgaagctcc tgggctataa cgaaaagcca ataaatctac
1501aaatgtttat tgggacagca gatgatcgat atttacgacc tcatgcattt taccaggtgc
1561atcgaatcac tgggaagaca gtcgctactg caagccaaga gataataatt gccagtacaa
1621aagttctgga aattccactt cttcctgaaa ataatatgtc agccagtatt gattgtgcag
1681gtattttgaa actccgcaat tcagatatag aacttcgaaa aggagaaact gatattggca
1741gaaagaatac tagagtacga cttgtgtttc gtgtacacat cccacagccc agtggaaaag
1801tcctttctct gcagatagcc tctatacccg ttgagtgctc ccagcggtct gctcaagaac
1861ttcctcatat tgagaagtac agtatcaaca gttgttctgt aaatggaggt catgaaatgg
1921ttgtgactgg atctaatttt cttccagaat ccaaaatcat ttttcttgaa aaaggacaag
1981atggacgacc tcagtgggag gtagaaggga agataatcag ggaaaaatgt caaggggctc
2041acattgtcct tgaagttcct ccatatcata acccagcagt tacagctgca gtgcaggtgc
2101acttttatct ttgcaatggc aagaggaaaa aaagccagtc tcaacgtttt acttatacac
2161cagttttgat gaagcaagaa cacagagaag agattgattt gtcttcagtt ccatctttgc
2221ctgtgcctca tcctgctcag acccagaggc cttcctctga ttcagggtgt tcacatgaca
2281gtgtactgtc aggacagaga agtttgattt gctccatccc acaaacatat gcatccatgg
2341tgacctcatc ccatctgcca cagttgcagt gtagagatga gagtgttagt aaagaacagc
2401atatgattcc ttctccaatt gtacaccagc cttttcaagt cacaccaaca cctcctgtgg
2461ggtcttccta tcagcctatg caaactaatg ttgtgtacaa tggaccaact tgtcttccta
2521ttaatgctgc ctctagtcaa gaatttgatt cagttttgtt tcagcaggat gcaactcttt
2581ctggtttagt gaatcttggc tgtcaaccac tgtcatccat accatttcat tcttcaaatt
2641caggctcaac aggacatctc ttagcccata cacctcattc tgtgcatacc ctgcctcatc
2701tgcaatcaat gggatatcat tgttcaaata caggacaaag atctctttct tctccagtgg
2761ctgaccagat tacaggtcag ccttcgtctc agttacaacc tattacatat ggtccttcac
2821attcagggtc tgctacaaca gcttccccag cagcttctca tcccttggct agttcaccgc
2881tttctgggcc accatctcct cagcttcagc ctatgcctta ccaatctcct agctcaggaa
2941ctgcctcatc accgtctcca gccaccagaa tgcattctgg acagcactca actcaagcac
3001aaagtacggg ccaggggggt ctttctgcac cttcatcctt aatatgtcac agtttgtgtg
3061atccagcgtc atttccacct gatggggcaa ctgtgagcat taaacctgaa ccagaagatc
3121gagagcctaa ctttgcaacc attggtctgc aggacatcac tttagatgat gtgaacgaga
3181taattgggag agacatgtcc cagatttctg tttcccaagg agcaggggtg agcaggcagg
3241ctcccctccc gagtcctgag tccctggatt taggaagatc tgatgggctc tacccaactt
3301tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
3361agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

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