Construct: ORF ccsbBroad304_01088
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006847.1_s304c1, BRDN0000405001
- Derived from:
- ccsbBroadEn_01088
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFATC3 (4775)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01088
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4775 | NFATC3 | nuclear factor of activated... | NM_173165.3 | 100% | 100% | |
| 2 | human | 4775 | NFATC3 | nuclear factor of activated... | NM_004555.4 | 97.5% | 96.4% | (many diffs) |
| 3 | human | 4775 | NFATC3 | nuclear factor of activated... | NM_173163.3 | 97.5% | 96.5% | (many diffs) |
| 4 | mouse | 18021 | Nfatc3 | nuclear factor of activated... | NM_010901.2 | 88.8% | 88.9% | (many diffs) |
| 5 | mouse | 18021 | Nfatc3 | nuclear factor of activated... | XM_006530753.2 | 87.7% | 86.5% | (many diffs) |
| 6 | mouse | 18021 | Nfatc3 | nuclear factor of activated... | XM_006530754.3 | 86.6% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3291
- ORF length:
- 3225
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tactgcaaac tgtggcgccc acgacgagct cgacttcaaa ctcgtctttg 121 gcgaggacgg ggcgccggcg ccgccgcccc cgggctcgcg gcctgcagat cttgagccag 181 atgattgtgc atccatttac atctttaatg tagatccacc tccatctact ttaaccacac 241 cactttgctt accacatcat ggattaccgt ctcactcttc tgttttgtca ccatcgtttc 301 agctccaaag tcacaaaaac tatgaaggaa cttgtgagat tcctgaatct aaatatagcc 361 cattaggtgg tcccaaaccc tttgagtgcc caagtattca aattacatct atctctccta 421 actgtcatca agaattagat gcacatgaag atgacctaca gataaatgac ccagaacggg 481 aatttttgga aaggccttct agagatcatc tctatcttcc tcttgagcca tcctaccggg 541 agtcttctct tagtcctagt cctgccagca gcatctcttc taggagttgg ttctctgatg 601 catcttcttg tgaatcgctt tcacatattt atgatgatgt ggactcagag ttgaatgaag 661 ctgcagcccg atttaccctt ggatcccctc tgacttctcc tggtggctct ccagggggct 721 gccctggaga agaaacttgg catcaacagt atggacttgg acactcatta tcacccaggc 781 aatctccttg ccactctcct agatccagtg tcactgatga gaattggctg agccccaggc 841 cagcctcagg accctcatca aggcccacat ccccctgtgg gaaacggagg cactccagtg 901 ctgaagtttg ttatgctggg tccctttcac cccatcactc acctgttcct tcacctggtc 961 actcccccag gggaagtgtg acagaagata cgtggctcaa tgcttctgtc catggtgggt 1021 caggccttgg ccctgcagtt tttccatttc agtactgtgt agagactgac atccctctca 1081 aaacaaggaa aacttctgaa gatcaagctg ccatactacc aggaaaatta gagctgtgtt 1141 cagatgacca agggagttta tcaccagccc gggagacttc aatagatgat ggccttggat 1201 ctcagtatcc tttaaagaaa gattcatgtg gtgatcagtt tctttcagtt ccttcaccct 1261 ttacctggag caaaccaaag cctggccaca cccctatatt tcgcacatct tcattacctc 1321 cactagactg gcctttacca gctcattttg gacaatgtga actgaaaata gaagtgcaac 1381 ctaaaactca tcatcgagcc cattatgaaa ctgaaggtag ccgaggggca gtaaaagcat 1441 ctactggggg acatcctgtt gtgaagctcc tgggctataa cgaaaagcca ataaatctac 1501 aaatgtttat tgggacagca gatgatcgat atttacgacc tcatgcattt taccaggtgc 1561 atcgaatcac tgggaagaca gtcgctactg caagccaaga gataataatt gccagtacaa 1621 aagttctgga aattccactt cttcctgaaa ataatatgtc agccagtatt gattgtgcag 1681 gtattttgaa actccgcaat tcagatatag aacttcgaaa aggagaaact gatattggca 1741 gaaagaatac tagagtacga cttgtgtttc gtgtacacat cccacagccc agtggaaaag 1801 tcctttctct gcagatagcc tctatacccg ttgagtgctc ccagcggtct gctcaagaac 1861 ttcctcatat tgagaagtac agtatcaaca gttgttctgt aaatggaggt catgaaatgg 1921 ttgtgactgg atctaatttt cttccagaat ccaaaatcat ttttcttgaa aaaggacaag 1981 atggacgacc tcagtgggag gtagaaggga agataatcag ggaaaaatgt caaggggctc 2041 acattgtcct tgaagttcct ccatatcata acccagcagt tacagctgca gtgcaggtgc 2101 acttttatct ttgcaatggc aagaggaaaa aaagccagtc tcaacgtttt acttatacac 2161 cagttttgat gaagcaagaa cacagagaag agattgattt gtcttcagtt ccatctttgc 2221 ctgtgcctca tcctgctcag acccagaggc cttcctctga ttcagggtgt tcacatgaca 2281 gtgtactgtc aggacagaga agtttgattt gctccatccc acaaacatat gcatccatgg 2341 tgacctcatc ccatctgcca cagttgcagt gtagagatga gagtgttagt aaagaacagc 2401 atatgattcc ttctccaatt gtacaccagc cttttcaagt cacaccaaca cctcctgtgg 2461 ggtcttccta tcagcctatg caaactaatg ttgtgtacaa tggaccaact tgtcttccta 2521 ttaatgctgc ctctagtcaa gaatttgatt cagttttgtt tcagcaggat gcaactcttt 2581 ctggtttagt gaatcttggc tgtcaaccac tgtcatccat accatttcat tcttcaaatt 2641 caggctcaac aggacatctc ttagcccata cacctcattc tgtgcatacc ctgcctcatc 2701 tgcaatcaat gggatatcat tgttcaaata caggacaaag atctctttct tctccagtgg 2761 ctgaccagat tacaggtcag ccttcgtctc agttacaacc tattacatat ggtccttcac 2821 attcagggtc tgctacaaca gcttccccag cagcttctca tcccttggct agttcaccgc 2881 tttctgggcc accatctcct cagcttcagc ctatgcctta ccaatctcct agctcaggaa 2941 ctgcctcatc accgtctcca gccaccagaa tgcattctgg acagcactca actcaagcac 3001 aaagtacggg ccaggggggt ctttctgcac cttcatcctt aatatgtcac agtttgtgtg 3061 atccagcgtc atttccacct gatggggcaa ctgtgagcat taaacctgaa ccagaagatc 3121 gagagcctaa ctttgcaacc attggtctgc aggacatcac tttagatgat gtgaacgaga 3181 taattgggag agacatgtcc cagatttctg tttcccaagg agcaggggtg agcaggcagg 3241 ctcccctccc gagtcctgag tccctggatt taggaagatc tgatgggctc tacccaactt 3301 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 3361 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta