Construct: ORF ccsbBroad304_01103
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013112.1_s304c1, BRDN0000406708
- Derived from:
- ccsbBroadEn_01103
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NONO (4841)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01103
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4841 | NONO | non-POU domain containing o... | NM_001145408.2 | 100% | 100% | |
| 2 | human | 4841 | NONO | non-POU domain containing o... | NM_001145409.2 | 100% | 100% | |
| 3 | human | 4841 | NONO | non-POU domain containing o... | NM_007363.5 | 100% | 100% | |
| 4 | human | 4841 | NONO | non-POU domain containing o... | NM_001145410.1 | 81.1% | 81.1% | 0_1ins267 |
| 5 | mouse | 53610 | Nono | non-POU-domain-containing, ... | NM_001252518.1 | 91.6% | 98.3% | (many diffs) |
| 6 | mouse | 53610 | Nono | non-POU-domain-containing, ... | NM_023144.2 | 91.6% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1479
- ORF length:
- 1413
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gagtaataaa acttttaact tggagaagca aaaccatact ccaagaaagc 121 atcatcaaca tcaccaccag cagcagcacc accagcagca acagcagcag ccgccaccac 181 cgccaatacc tgcaaatggg caacaggcca gcagccaaaa tgaaggcttg actattgacc 241 tgaagaattt tagaaaacca ggagagaaga ccttcaccca acgaagccgt ctttttgtgg 301 gaaatcttcc tcccgacatc actgaggaag aaatgaggaa actatttgag aaatatggaa 361 aggcaggcga agtcttcatt cataaggata aaggatttgg ctttatccgc ttggaaaccc 421 gaaccctagc ggagattgcc aaagtggagc tggacaatat gccactccgt ggaaagcagc 481 tgcgtgtgcg ctttgcctgc catagtgcat cccttacagt tcgaaacctt cctcagtatg 541 tgtccaacga actgctggaa gaagcctttt ctgtgtttgg ccaggtagag agggctgtag 601 tcattgtgga tgatcgagga aggccctcag gaaaaggcat tgttgagttc tcagggaagc 661 cagctgctcg gaaagctctg gacagatgca gtgaaggctc cttcctgcta accacatttc 721 ctcgtcctgt gactgtggag cccatggacc agttagatga tgaagaggga cttccagaga 781 agctggttat aaaaaaccag caatttcaca aggaacgaga gcagccaccc agatttgcac 841 agcctggctc ctttgagtat gaatatgcca tgcgctggaa ggcactcatt gagatggaga 901 agcagcagca ggaccaagtg gaccgcaaca tcaaggaggc tcgtgagaag ctggagatgg 961 agatggaagc tgcacgccat gagcaccagg tcatgctaat gagacaggat ttgatgaggc 1021 gccaagaaga acttcggagg atggaagagc tgcacaacca agaggtgcaa aaacgaaagc 1081 aactggagct caggcaggag gaagagcgca ggcgccgtga agaagagatg cggcggcagc 1141 aagaagaaat gatgcggcga cagcaggaag gattcaaggg aaccttccct gatgcgagag 1201 agcaggagat tcggatgggt cagatggcta tgggaggtgc tatgggcata aacaacagag 1261 gtgccatgcc ccctgctcct gtgccagctg gtaccccagc tcctccagga cctgccacta 1321 tgatgccgga tggaactttg ggattgaccc caccaacaac tgaacgcttt ggtcaggctg 1381 ctacaatgga aggaattggg gcaattggtg gaactcctcc tgcattcaac cgtgcagctc 1441 ctggagctga atttgcccca aacaaacgtc gccgatacta cccaactttc ttgtacaaag 1501 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1561 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta