Transcript: Mouse NM_001252518.1

Mus musculus non-POU-domain-containing, octamer binding protein (Nono), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-18
Taxon:
Mus musculus (mouse)
Gene:
Nono (53610)
Length:
2465
CDS:
179..1600

Additional Resources:

NCBI RefSeq record:
NM_001252518.1
NBCI Gene record:
Nono (53610)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001252518.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000295777 ACACGAACCCTAGCGGAAATT pLKO_005 536 CDS 100% 13.200 18.480 N Nono n/a
2 TRCN0000102629 CGTCTCTTTGTGGGCAATCTT pLKO.1 407 CDS 100% 5.625 7.875 N Nono n/a
3 TRCN0000102625 CGGTTGTGTTATATCTCATAT pLKO.1 2084 3UTR 100% 13.200 9.240 N Nono n/a
4 TRCN0000288456 CGGTTGTGTTATATCTCATAT pLKO_005 2084 3UTR 100% 13.200 9.240 N Nono n/a
5 TRCN0000295719 TGAGCACCAGGTTATGCTAAT pLKO_005 1099 CDS 100% 10.800 7.560 N Nono n/a
6 TRCN0000102626 CCCACCAACAACTGAACGTTT pLKO.1 1468 CDS 100% 4.950 3.465 N Nono n/a
7 TRCN0000102627 GCCAGAATGAAGGCTTGACTA pLKO.1 333 CDS 100% 4.950 3.465 N Nono n/a
8 TRCN0000288455 GCCAGAATGAAGGCTTGACTA pLKO_005 333 CDS 100% 4.950 3.465 N Nono n/a
9 TRCN0000074558 GCCAGAATTCTACCCTGGAAA pLKO.1 1659 3UTR 100% 0.000 0.000 N NONO n/a
10 TRCN0000286693 GCCAGAATTCTACCCTGGAAA pLKO_005 1659 3UTR 100% 0.000 0.000 N NONO n/a
11 TRCN0000102628 GCTGCAACAATGGAAGGAATT pLKO.1 1496 CDS 100% 0.000 0.000 N Nono n/a
12 TRCN0000288457 GCTGCAACAATGGAAGGAATT pLKO_005 1496 CDS 100% 0.000 0.000 N Nono n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001252518.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01103 pDONR223 100% 91.6% 98.3% None (many diffs) n/a
2 ccsbBroad304_01103 pLX_304 0% 91.6% 98.3% V5 (many diffs) n/a
3 ccsbBroadEn_11000 pDONR223 100% 66.3% 71.4% None (many diffs) n/a
4 ccsbBroad304_11000 pLX_304 0% 66.3% 71.4% V5 (many diffs) n/a
5 TRCN0000469986 CAGCTCGTTGATTAAAGCAGTTTT pLX_317 38.4% 66.3% 71.4% V5 (many diffs) n/a
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