Construct: ORF ccsbBroad304_01138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003525.1_s304c1, BRDN0000400157
- Derived from:
- ccsbBroadEn_01138
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P4HB (5034)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01138
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5034 | P4HB | prolyl 4-hydroxylase subuni... | NM_000918.4 | 100% | 100% | |
| 2 | human | 5034 | P4HB | prolyl 4-hydroxylase subuni... | XM_024450777.1 | 91.4% | 69.6% | 1178_1305del;1638_1639insTGAAAGATGAACTG |
| 3 | human | 5034 | P4HB | prolyl 4-hydroxylase subuni... | XR_002958014.1 | 54.9% | 1_64del;1423_1752del;1919_2774del | |
| 4 | mouse | 18453 | P4hb | prolyl 4-hydroxylase, beta ... | NM_011032.2 | 86.4% | 93.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gcgccgcgct ctgctgtgcc tggccgtggc cgccctggtg cgcgccgacg 121 cccccgagga ggaggaccac gtcctggtgc tgcggaaaag caacttcgcg gaggcgctgg 181 cggcccacaa gtacctgctg gtggagttct atgccccttg gtgtggccac tgcaaggctc 241 tggcccctga gtatgccaaa gccgctggga agctgaaggc agaaggttcc gagatcaggt 301 tggccaaggt ggacgccacg gaggagtctg acctggccca gcagtacggc gtgcgcggct 361 atcccaccat caagttcttc aggaatggag acacggcttc ccccaaggaa tatacagctg 421 gcagagaggc tgatgacatc gtgaactggc tgaagaagcg cacgggcccg gctgccacca 481 ccctgcctga cggcgcagct gcagagtcct tggtggagtc cagcgaggtg gctgtcatcg 541 gcttcttcaa ggacgtggag tcggactctg ccaagcagtt tttgcaggca gcagaggcca 601 tcgatgacat accatttggg atcacttcca acagtgacgt gttctccaaa taccagctcg 661 acaaagatgg ggttgtcctc tttaagaagt ttgatgaagg ccggaacaac tttgaagggg 721 aggtcaccaa ggagaacctg ctggacttta tcaaacacaa ccagctgccc cttgtcatcg 781 agttcaccga gcagacagcc ccgaagattt ttggaggtga aatcaagact cacatcctgc 841 tgttcttgcc caagagtgtg tctgactatg acggcaaact gagcaacttc aaaacagcag 901 ccgagagctt caagggcaag atcctgttca tcttcatcga cagcgaccac accgacaacc 961 agcgcatcct cgagttcttt ggcctgaaga aggaagagtg cccggccgtg cgcctcatca 1021 ccctggagga ggagatgacc aagtacaagc ccgaatcgga ggagctgacg gcagagagga 1081 tcacagagtt ctgccaccgc ttcctggagg gcaaaatcaa gccccacctg atgagccagg 1141 agctgccgga ggactgggac aagcagcctg tcaaggtgct tgttgggaag aactttgaag 1201 acgtggcttt tgatgagaaa aaaaacgtct ttgtggagtt ctatgcccca tggtgtggtc 1261 actgcaaaca gttggctccc attTGGGATA AACTGGGAGA GACGTACAAG GACCATGAGA 1321 ACATCGTCAT CGCCAAGATG GACTCGACTG CCAACGAGGT GGAGGCCGTC AAAGTGCACA 1381 GCTTCCCCAC ACTCAAGTTC TTTCCTGCCA GTGCCGACAG GACGGTCATT GATTACAACG 1441 GGGAACGCAC GCTGGATGGT TTTAAGAAAT TCCTGGAGAG CGGTGGCCAG GATGGGGCAG 1501 GGGATGATGA CGATCTCGAG GACCTGGAAG AAGCAGAGGA GCCAGACATG GAGGAAGACG 1561 ATGATCAGAA AGCTGTGAAA GATGAACTGT ACccaacttc cttgtacaaa gtggttggta 1621 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1681 ggtggcgcgt taagtcgaca atcaacctct ggatta