Construct: ORF ccsbBroad304_01154
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012473.1_s304c1, BRDN0000404974
- Derived from:
- ccsbBroadEn_01154
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCP4 (5121)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01154
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5121 | PCP4 | Purkinje cell protein 4 | NM_006198.3 | 100% | 100% | |
| 2 | mouse | 18546 | Pcp4 | Purkinje cell protein 4 | NM_008791.3 | 89.2% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 252
- ORF length:
- 186
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tgagcgacaa ggtgctgggg caaccaatgg aaaagacaag acatctggtg 121 aaaatgatgg acagaagaaa gttcaagaag aatttgacat tgacatggat gcaccagaga 181 cagaacgtgc agcggtggcc attcagtctc agttcagaaa attccagaag aagaaggctg 241 ggtctcagtc ctacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 301 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 361 caatcaacct ctggatta