Construct: ORF ccsbBroad304_01157

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012773.1_s304c1, BRDN0000461162
Derived from:
ccsbBroadEn_01157
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCYT1A (5130)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01157
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5130 PCYT1A phosphate cytidylyltransfer... NM_001312673.2 100% 100%
2 human 5130 PCYT1A phosphate cytidylyltransfer... NM_005017.4 100% 100%
3 mouse 13026 Pcyt1a phosphate cytidylyltransfer... NM_001163159.1 89.7% 96.1% (many diffs)
4 mouse 13026 Pcyt1a phosphate cytidylyltransfer... NM_001163160.1 89.7% 96.1% (many diffs)
5 mouse 13026 Pcyt1a phosphate cytidylyltransfer... NM_009981.4 89.7% 96.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1167
ORF length:
1101
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tgcacagtgt tcagccaagg tcaatgcaag gaagaggaga aaagaggcgc
121ccggacccaa cggggcaaca gaagaagatg gggttccttc caaagtgcag cgctgtgcag
181tgggcttacg gcaaccagct cctttttctg atgaaattga agttgacttt agtaagccct
241atgtcagggt aactatggaa gaagccagca gaggaactcc ttgtgagcga cctgtgagag
301tttatgccga tggaatattt gacttatttc actctggtca cgcccgagct ctgatgcaag
361cgaagaacct tttccctaat acgtacctca ttgtgggagt ttgcagtgat gagctcacac
421acaacttcaa aggcttcacg gtgatgaacg agaatgagcg ctatgacgca gtccagcact
481gccgctacgt ggatgaggtg gtgaggaatg cgccctggac gctgacaccc gagttcctgg
541ccgaacaccg gattgatttt gtagcccatg atgatattcc ttattcatct gctggcagtg
601atgatgttta taagcacatc aaggaggcag gcatgtttgc tccaacacag aggacagaag
661gtatctccac atcagacatc atcacccgaa ttgtgcggga ttatgatgtg tatgcgaggc
721ggaacctgca gaggggctac acagcaaagg agctcaatgt cagctttatc aacgagaaga
781aataccactt gcaggagagg gttgacaaag taaagaagaa agtgaaagat gtggaggaaa
841agtcaaaaga atttgttcag aaggtggagg aaaaaagcat tgacctcatt cagaagtggg
901aggagaagtc ccgagaattc attggaagtt ttctggaaat gtttggtccg gaaggagcac
961tgaaacatat gctgaaagag gggaagggcc ggatgctgca ggccatcagc ccgaagcaga
1021gccccagcag cagccctact cgcgagcgct ccccctcccc ctctttccga tggcccttct
1081ccggcaagac ttccccacct tgctccccag caaatctctc caggcacaag gctgcagcct
1141atgatatcag tgaggatgaa gaagactacc caactttctt gtacaaagtg gttggtaagc
1201ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1261ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)