Construct: ORF ccsbBroad304_01173

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000747.1_s304c1, BRDN0000404777
Derived from:
ccsbBroadEn_01173
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PEX7 (5191)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01173
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5191 PEX7 peroxisomal biogenesis fact... NM_000288.4 100% 100%
2 human 5191 PEX7 peroxisomal biogenesis fact... XM_005267019.4 87.5% 86.6% (many diffs)
3 human 5191 PEX7 peroxisomal biogenesis fact... XM_006715502.2 69.6% 69.6% 336_337ins294
4 human 5191 PEX7 peroxisomal biogenesis fact... XM_017010934.2 61.6% 53.9% 524_525ins277;597_598ins95
5 mouse 18634 Pex7 peroxisomal biogenesis fact... NM_008822.2 87.3% 90.4% (many diffs)
6 mouse 18634 Pex7 peroxisomal biogenesis fact... NM_001161825.1 79.5% 82.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1035
ORF length:
969
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tgcggtgtgc ggtggagcgg cgcggatgct gcggacgccg ggacgccacg
121gctacgccgc cgagttctcc ccgtacctgc cgggccgcct ggcctgcgcc accgcgcagc
181actacggcat cgcgggctgt ggaaccctac taatattgga tccagatgaa gctgggctaa
241ggctttttag aagctttgac tggaatgatg gtttgtttga tgtgacttgg agtgagaaca
301acgaacatgt cctcatcacc tgtagtggcg atggctcgct gcagctctgg gacactgcca
361aagctgcagg gccactgcaa gtctataaag aacacgctca ggaggtgtat agtgttgatt
421ggagccaaac cagaggtgaa cagcttgtgg tgtctggctc atgggatcaa actgtcaaat
481tgtgggatcc aactgttgga aagtctctgt gcacctttag aggccatgaa agtattattt
541atagcacaat ctggtctccc cacatccctg gttgttttgc ttcagcctca ggtgatcaga
601ctctgagaat atgggatgtg aaggcagcag gagtaagaat cgtgattcct gcacatcagg
661cagaaatctt gagttgtgac tggtgtaaat acaatgagaa tttgctggtg accggggcgg
721ttgactgtag tttgagaggc tgggacttaa ggaatgtacg acaaccagtg tttgaacttc
781ttggtcatac ctatgctatt aggagggtga aattttcacc atttcatgct tctgtgctgg
841cctcttgctc gtatgatttt actgtaagat tctggaactt ttcaaagcct gactctcttc
901ttgaaacagt ggagcatcat acagagttta cttgtggttt agacttcagt cttcagagcc
961ccactcaggt ggctgactgt tcttgggatg aaacaataaa gatctatgac cctgcttgtc
1021ttactattcc tgcttgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1081ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1141cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)