Construct: ORF ccsbBroad304_01296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002806.1_s304c1, BRDN0000402612
- Derived from:
- ccsbBroadEn_01296
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRM2 (5620)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01296
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5620 | PRM2 | protamine 2 | NM_002762.4 | 100% | 100% | |
| 2 | human | 5620 | PRM2 | protamine 2 | NM_001286359.2 | 85.9% | 82.8% | (many diffs) |
| 3 | human | 5620 | PRM2 | protamine 2 | NM_001286358.2 | 79.5% | 71.3% | 269_270insAGGCTGC;300_369del |
| 4 | human | 5620 | PRM2 | protamine 2 | NM_001286357.2 | 75.1% | 74.5% | 230A>G;231_232ins75 |
| 5 | human | 5620 | PRM2 | protamine 2 | NM_001286356.2 | 72.8% | 65.7% | 270_313del;351_420del |
| 6 | human | 5620 | PRM2 | protamine 2 | NR_104428.2 | 51.4% | 1_110del;417_595del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 372
- ORF length:
- 306
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ccgataccgc gtgaggagcc tgagcgaacg ctcgcacgag gtgtacaggc 121 agcagttgca tgggcaagag caaggacacc acggccaaga ggagcaaggg ctgagcccgg 181 agcacgtcga ggtctacgag aggacccatg gccagtctca ctataggcgc agacactgct 241 ctcgaaggag gctgcaccgg atccacaggc ggcagcatcg ctcctgcaga aggcgcaaaa 301 gacgctcctg caggcaccgg aggaggcatc gcagaggctg cagaaccagg aagagaacat 361 gcagaaggca ctacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 421 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 481 caatcaacct ctggatta