Construct: ORF ccsbBroad304_01349

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013667.1_s304c1, BRDN0000411895
Derived from:
ccsbBroadEn_01349
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NECTIN1 (5818)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01349
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5818 NECTIN1 nectin cell adhesion molecu... NM_002855.5 100% 100%
2 human 5818 NECTIN1 nectin cell adhesion molecu... NM_203285.2 76.5% 70.3% (many diffs)
3 human 5818 NECTIN1 nectin cell adhesion molecu... NM_203286.2 65.7% 65.3% (many diffs)
4 mouse 58235 Nectin1 nectin cell adhesion molecu... NM_021424.2 90.2% 94.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1620
ORF length:
1551
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggctcggatg gggcttgcgg gcgccgctgg acgctggtgg ggactcgctc
121tcggcttgac cgcattcttc ctcccaggcg tccactccca ggtggtccag gtgaacgact
181ccatgtatgg cttcatcggc acagacgtgg ttctgcactg cagctttgcc aacccgcttc
241ccagcgtgaa gatcacccag gtcacatggc agaagtccac caatggctcc aagcagaacg
301tggccatcta caacccatcc atgggcgtgt ccgtgctggc tccctaccgc gagcgtgtgg
361aattcctgcg gccctccttc accgatggca ctatccgcct ctcccgcctg gagctggagg
421atgagggtgt ctacatctgc gagtttgcta ccttccctac gggcaatcga gaaagccagc
481tcaatctcac ggtgatggcc aaacccacca attggataga gggtacccag gcagtgcttc
541gagccaagaa ggggcaggat gacaaggtcc tggtggccac ctgcacctca gccaatggga
601agcctcccag tgtggtatcc tgggaaactc ggttaaaagg tgaggcagag taccaggaga
661tccggaaccc caatggcaca gtgacggtca tcagccgcta ccgcctggtg cccagcaggg
721aagcccacca gcagtccttg gcctgcatcg tcaactacca catggaccgc ttcaaggaaa
781gcctcactct caacgtgcag tatgagcctg aggtaaccat tgaggggttt gatggcaact
841ggtacctgca gcggatggac gtgaagctca cctgcaaagc tgatgctaac cccccagcca
901ctgagtacca ctggaccacg ctaaatggct ctctccccaa gggtgtggag gcccagaaca
961gaaccctctt cttcaaggga cccatcaact acagcctggc agggacctac atctgtgagg
1021ccaccaaccc catcggtaca cgctcaggcc aggtggaggt caatatcaca gaattcccct
1081acaccccgtc tcctcccgaa catgggcggc gcgccgggcc ggtgcccacg gccatcattg
1141ggggcgtggc ggggagcatc ctgctggtgt tgattgtggt cggcgggatc gtggtcgccc
1201tgcgtcggcg ccggcacacc ttcaagggtg actacagcac caagaagcac gtgtatggca
1261acggctacag caaggcaggc atcccccagc accacccacc aatggcacag aacctgcagt
1321accccgacga ctcagacgac gagaagaagg ccggcccact gggtggaagc agctatgagg
1381aggaggagga ggaggaggag ggcggtggag ggggcgagcg caaggtgggc ggcccccacc
1441ccaaatatga cgaggacgcc aagcggccct acttcaccgt ggatgaggcc gaggcccgtc
1501aggacggcta cggggaccgg actctgggct accagtacga ccctgagcag ctggacttgg
1561ctgagaacat ggtttctcag aacgacgggt ctttcatttc caagaaggag tggtacgtgt
1621tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1681attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1741ggatta

Download FASTA (ORF) (Full)