Construct: ORF ccsbBroad304_01378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015832.1_s304c1, BRDN0000399248
- Derived from:
- ccsbBroadEn_01378
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASA1 (5921)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01378
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5921 | RASA1 | RAS p21 protein activator 1 | NM_002890.3 | 100% | 100% | |
| 2 | human | 5921 | RASA1 | RAS p21 protein activator 1 | XM_011543525.2 | 97.2% | 97.2% | 2603_2604ins87 |
| 3 | human | 5921 | RASA1 | RAS p21 protein activator 1 | NM_022650.2 | 82.9% | 82.8% | 0_1ins531;2_3delTGinsCT;6_8delGGGinsCCA |
| 4 | human | 5921 | RASA1 | RAS p21 protein activator 1 | XM_011543527.3 | 51.4% | 51.1% | 1609_1610insGGCCAAACT;1615_1616delAGinsCA;1617_1618ins1515 |
| 5 | mouse | 218397 | Rasa1 | RAS p21 protein activator 1 | NM_145452.3 | 90.6% | 95.1% | (many diffs) |
| 6 | mouse | 218397 | Rasa1 | RAS p21 protein activator 1 | XM_011244518.2 | 77.1% | 81% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3207
- ORF length:
- 3141
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaagg 61 ttggcatgat ggcggccgag gccggcagtg aggagggcgg cccggtaaca gccggagctg 121 gaggaggcgg cgcggcagcg ggctccagtg cctatcccgc agtgtgtcgg gtgaagatac 181 ccgcggccct gcctgtggca gccgccccct atcctgggct ggtggagacc ggagtggctg 241 gaactctggg tggcggagcc gctttggggt cagagttcct aggagccggg tctgtggcag 301 gggcactggg gggagctgga ctgacagggg gaggtactgc tgctggcgta gctggtgctg 361 ctgctggcgt ggccggtgct gctgttgctg gacctagtgg agacatggct ctcaccaaac 421 tgcccacttc gttgcttgct gagactctcg ggccaggcgg cggttttccc cctctgcccc 481 ctccccctta cctgccccct ttgggggcgg gcctcgggac agtggacgaa ggtgactctc 541 tggatggacc agaatacgag gaggaagagg tggccatacc gttgaccgct cctccaacta 601 accagtggta tcacggaaaa cttgacagaa cgatagcaga agaacgcctc aggcaggcag 661 ggaagtctgg cagttatctt ataagagaga gtgatcggag gccagggtcc tttgtacttt 721 catttcttag ccagatgaat gttgtcaacc attttaggat tattgctatg tgtggagatt 781 actacattgg tggaagacgt ttttcttcac tgtcagacct aataggttat tacagtcatg 841 tttcttgttt gcttaaagga gaaaaattac tttacccagt tgcaccacca gagccagtag 901 aagatagaag gcgtgtacga gctattctac cttacacaaa agtaccagac actgatgaaa 961 taagtttctt aaaaggagat atgttcattg ttcataatga attagaagat ggatggatgt 1021 gggttacaaa tttaagaaca gatgaacaag gccttattgt tgaagaccta gtagaagagg 1081 tgggccggga agaagatcca catgaaggaa aaatatggtt ccatgggaag atttccaaac 1141 aggaagctta taatttacta atgacagttg gtcaagtctg cagttttctt gtgaggccct 1201 cagataatac tcctggcgat tattcacttt atttccggac caatgaaaat attcagcgat 1261 ttaaaatatg tccaacgcca aacaatcagt ttatgatggg aggccggtat tataacagca 1321 ttggggacat catagatcac tatcgaaaag aacagattgt tgaaggatat tatcttaagg 1381 aacctgtacc aatgcaggat caagaacaag tactcaatga cacagtggat ggcaaggaaa 1441 tctataatac catccgtcgt aaaacaaagg atgcctttta taaaaacatt gttaagaaag 1501 gttatcttct gaaaaagggc aaaggaaaac gttggaaaaa tttatatttt atcttagagg 1561 gtagtgatgc ccaacttatt tattttgaaa gcgaaaaacg agctaccaaa ccaaaaggat 1621 taatagatct cagtgtatgt tctgtctatg tcgttcatga tagtctcttt ggcaggccaa 1681 actgttttca gatagtagtt cagcacttta gtgaagaaca ttacatcttt tactttgcag 1741 gagaaactcc agaacaagca gaggattgga tgaaaggtct gcaggcattt tgcaatttac 1801 ggaaaagtag tccagggaca tccaataaac gccttcgtca ggtcagcagc cttgttttac 1861 atattgaaga agcccataaa ctcccagtaa aacattttac taatccatat tgtaacatct 1921 acctgaatag tgtccaagta gcaaaaactc atgcaaggga agggcaaaac ccagtatggt 1981 cagaagagtt tgtctttgat gatcttcctc ctgacatcaa tagatttgaa ataactctta 2041 gtaataaaac aaagaaaagc aaagatcctg atatcttatt tatgcgctgc cagttgagcc 2101 gattacagaa agggcatgcc acagatgaat ggtttctgct cagctcccat ataccattaa 2161 aaggtattga accagggtcc ctgcgtgttc gagcacgata ctctatggaa aaaatcatgc 2221 cagaagaaga gtacagtgaa tttaaagagc ttatactgca aaaggaactt catgtagtct 2281 atgctttatc acatgtatgt ggacaagacc gaacactact ggccagcatc ctactgagga 2341 tttttcttca cgaaaagctt gaatcgttgt tgttatgcac actaaatgac agagaaataa 2401 gcatggaaga tgaagccact accctatttc gagccacaac acttgcaagc accttgatgg 2461 agcagtatat gaaagccact gctacacagt ttgttcatca tgctttgaaa gactctattt 2521 taaagataat ggaaagcaag cagtcttgtg agttaagtcc atcaaagtta gaaaaaaatg 2581 aagatgtgaa cactaattta acacacctat tgaacatact ttcagagctt gtggagaaaa 2641 tattcatggc ttcagaaata cttccaccga cattgagata tatttatggg tgtttacaga 2701 aatctgttca gcataagtgg cctacaaata ccaccatgag aacaagagtt gttagtggtt 2761 ttgtttttct tcgactcatc tgtcctgcca tcctgaatcc acggatgttc aatatcatct 2821 cagattctcc atctcctatt gctgcaagaa cactgatatt agtggctaaa tctgtgcaga 2881 acttagcaaa tcttgtggaa tttggagcta aggagcccta catggaaggt gtcaatccat 2941 tcatcaaaag caacaaacat cgtatgatca tgtttttaga tgaacttggg aatgtacctg 3001 aacttccgga cactacagag cattctagaa cggacctgtc ccgtgattta gcagcattgc 3061 atgagatttg cgtggctcat tcagatgaac ttcgaacgct cagtaatgag cgtggtgcac 3121 agcagcacgt attgaaaaag cttctggcta taacagaact gcttcaacaa aaacaaaacc 3181 agtatacaaa aaccaatgat gtcaggtacc caactttctt gtacaaagtg gttggtaagc 3241 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3301 ggcgcgttaa gtcgacaatc aacctctgga tta