Construct: ORF ccsbBroad304_01415

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014657.1_s304c1, BRDN0000461576
Derived from:
ccsbBroadEn_01415
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPE65 (6121)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01415
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6121 RPE65 retinoid isomerohydrolase R... NM_000329.3 100% 100%
2 human 6121 RPE65 retinoid isomerohydrolase R... XM_017002027.1 82.7% 82.7% 0_1ins276
3 mouse 19892 Rpe65 retinal pigment epithelium 65 NM_029987.2 89.9% 93.6% (many diffs)
4 mouse 19892 Rpe65 retinal pigment epithelium 65 XM_006501165.2 89.7% 93.4% (many diffs)
5 mouse 19892 Rpe65 retinal pigment epithelium 65 XR_001783668.1 40.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1668
ORF length:
1599
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtctatccag gttgagcatc ctgctggtgg ttacaagaaa ctgtttgaaa
121ctgtggagga actgtcctcg ccgctcacag ctcatgtaac aggcaggatc cccctctggc
181tcaccggcag tctccttcga tgtgggccag gactctttga agttggatct gagccatttt
241accacctgtt tgatgggcaa gccctcctgc acaagtttga ctttaaagaa ggacatgtca
301cataccacag aaggttcatc cgcactgatg cttacgtacg ggcaatgact gagaaaagga
361tcgtcataac agaatttggc acctgtgctt tcccagatcc ctgcaagaat atattttcca
421ggtttttttc ttactttcga ggagtagagg ttactgacaa tgcccttgtt aatgtctacc
481cagtggggga agattactac gcttgcacag agaccaactt tattacaaag attaatccag
541agaccttgga gacaattaag caggttgatc tttgcaacta tgtctctgtc aatggggcca
601ctgctcaccc ccacattgaa aatgatggaa ccgtttacaa tattggtaat tgctttggaa
661aaaatttttc aattgcctac aacattgtaa agatcccacc actgcaagca gacaaggaag
721atccaataag caagtcagag atcgttgtac aattcccctg cagtgaccga ttcaagccat
781cttacgttca tagttttggt ctgactccca actatatcgt ttttgtggag acaccagtca
841aaattaacct gttcaagttc ctttcttcat ggagtctttg gggagccaac tacatggatt
901gttttgagtc caatgaaacc atgggggttt ggcttcatat tgctgacaaa aaaaggaaaa
961agtacctcaa taataaatac agaacttctc ctttcaacct cttccatcac atcaacacct
1021atgaagacaa tgggtttctg attgtggatc tctgctgctg gaaaggattt gagtttgttt
1081ataattactt atatttagcc aatttacgtg agaactggga agaggtgaaa aaaaatgcca
1141gaaaggctcc ccaacctgaa gttaggagat atgtacttcc tttgaatatt gacaaggctg
1201acacaggcaa gaatttagtc acgctcccca atacaactgc cactgcaatt ctgtgcagtg
1261acgagactat ctggctggag cctgaagttc tcttttcagg gcctcgtcaa gcatttgagt
1321ttcctcaaat caattaccag aagtattgtg ggaaacctta cacatatgcg tatggacttg
1381gcttgaatca ctttgttcca gataggctct gtaagctgaa tgtcaaaact aaagaaactt
1441gggtttggca agagcctgat tcatacccat cagaacccat ctttgtttct cacccagatg
1501ccttggaaga agatgatggt gtagttctga gtgtggtggt gagcccagga gcaggacaaa
1561agcctgctta tctcctgatt ctgaatgcca aggacttaag tgaagttgcc cgggctgaag
1621tggagattaa catccctgtc acctttcatg gactgttcaa aaaatctttg ccaactttct
1681tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1741aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)