Construct: ORF ccsbBroad304_01427
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004661.1_s304c1, BRDN0000408100
- Derived from:
- ccsbBroadEn_01427
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPL24 (6152)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01427
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6152 | RPL24 | ribosomal protein L24 | NM_000986.4 | 100% | 100% | |
| 2 | mouse | 68193 | Rpl24 | ribosomal protein L24 | NM_024218.4 | 91.7% | 100% | (many diffs) |
| 3 | mouse | 668829 | Gm9385 | predicted pseudogene 9385 | XR_379934.3 | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tcgccatgaa ggtcgagctg tgcagtttta gcgggtacaa gatctacccc ggacacggga 121 ggcgctacgc caggaccgac gggaaggttt tccagtttct taatgcgaaa tgcgagtcgg 181 ctttcctttc caagaggaat cctcggcaga taaactggac tgtcctctac agaaggaagc 241 acaaaaaggg acagtcggaa gaaattcaaa agaaaagaac ccgccgagca gtcaaattcc 301 agagggccat tactggtgca tctcttgctg atataatggc caagaggaat cagaaacctg 361 aagttagaaa ggctcaacga gaacaagcta tcagggctgc taaggaagca aaaaaggcta 421 agcaagcatc taaaaagact gcaatggctg ctgctaaggc acctacaaag gcagcaccta 481 agcaaaagat tgtgaagcct gtgaaagttt cagctccccg agttggtgga aaacgctaaa 541 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 601 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 661 tta