Construct: ORF ccsbBroad304_01466
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009267.1_s304c1, BRDN0000410545
- Derived from:
- ccsbBroadEn_01466
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RRM2 (6241)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01466
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6241 | RRM2 | ribonucleotide reductase re... | NM_001034.4 | 100% | 100% | |
| 2 | human | 6241 | RRM2 | ribonucleotide reductase re... | NM_001165931.1 | 86.6% | 86.6% | 1_180del |
| 3 | human | 6241 | RRM2 | ribonucleotide reductase re... | NR_164157.1 | 51.5% | (many diffs) | |
| 4 | mouse | 20135 | Rrm2 | ribonucleotide reductase M2 | NM_009104.2 | 88% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1233
- ORF length:
- 1167
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct ctccctccgt gtcccgctcg cgcccatcac ggacccgcag cagctgcagc 121 tctcgccgct gaaggggctc agcttggtcg acaaggagaa cacgccgccg gccctgagcg 181 ggacccgcgt cctggccagc aagaccgcga ggaggatctt ccaggagccc acggagccga 241 aaactaaagc agctgccccc ggcgtggagg atgagccgct gctgagagaa aacccccgcc 301 gctttgtcat cttccccatc gagtaccatg atatctggca gatgtataag aaggcagagg 361 cttccttttg gaccgccgag gaggtggacc tctccaagga cattcagcac tgggaatccc 421 tgaaacccga ggagagatat tttatatccc atgttctggc tttctttgca gcaagcgatg 481 gcatagtaaa tgaaaacttg gtggagcgat ttagccaaga agttcagatt acagaagccc 541 gctgtttcta tggcttccaa attgccatgg aaaacataca ttctgaaatg tatagtcttc 601 ttattgacac ttacataaaa gatcccaaag aaagggaatt tctcttcaat gccattgaaa 661 cgatgccttg tgtcaagaag aaggcagact gggccttgcg ctggattggg gacaaagagg 721 ctacctatgg tgaacgtgtt gtagcctttg ctgcagtgga aggcattttc ttttccggtt 781 cttttgcgtc gatattctgg ctcaagaaac gaggactgat gcctggcctc acattttcta 841 atgaacttat tagcagagat gagggtttac actgtgattt tgcttgcctg atgttcaaac 901 acctggtaca caaaccatcg gaggagagag taagagaaat aattatcaat gctgttcgga 961 tagaacagga gttcctcact gaggccttgc ctgtgaagct cattgggatg aattgcactc 1021 taatgaagca atacattgag tttgtggcag acagacttat gctggaactg ggttttagca 1081 aggttttcag agtagagaac ccatttgact ttatggagaa tatttcactg gaaggaaaga 1141 ctaacttctt tgagaagaga gtaggcgagt atcagaggat gggagtgatg tcaagtccaa 1201 cagagaattc ttttaccttg gatgctgact tctacccaac tttcttgtac aaagtggttg 1261 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1321 accggtggcg cgttaagtcg acaatcaacc tctggatta