Construct: ORF ccsbBroad304_01498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002184.1_s304c1, BRDN0000398704
- Derived from:
- ccsbBroadEn_01498
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL5 (6352)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01498
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6352 | CCL5 | C-C motif chemokine ligand 5 | NM_002985.3 | 100% | 100% | |
| 2 | human | 6352 | CCL5 | C-C motif chemokine ligand 5 | NM_001278736.2 | 59% | 41.1% | 189_270del;356_462del |
| 3 | mouse | 20304 | Ccl5 | chemokine (C-C motif) ligand 5 | NM_013653.3 | 85.3% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 339
- ORF length:
- 273
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggtctccgcg gcagccctcg ctgtcatcct cattgctact gccctctgcg 121 ctcctgcatc tgcctcccca tattcctcgg acaccacacc ctgctgcttt gcctacattg 181 cccgcccact gccccgtgcc cacatcaagg agtatttcta caccagtggc aagtgctcca 241 acccagcagt cgtctttgtc acccgaaaga accgccaagt gtgtgccaac ccagagaaga 301 aatgggttcg ggagtacatc aactctttgg agatgagcta cccaactttc ttgtacaaag 361 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 421 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta