Construct: ORF ccsbBroad304_01501
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015595.1_s304c1, BRDN0000407775
- Derived from:
- ccsbBroadEn_01501
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL14 (6358)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01501
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6358 | CCL14 | C-C motif chemokine ligand 14 | NM_032963.4 | 100% | 100% | |
| 2 | human | 6358 | CCL14 | C-C motif chemokine ligand 14 | NM_032962.4 | 85.3% | 84.4% | 80_127del |
| 3 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027922.3 | 19.7% | 1_989del;1269_1416del | |
| 4 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027921.3 | 19% | 1_989del;1069_1116del;1317_1464del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 345
- ORF length:
- 279
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatctccgtg gctgccattc ccttcttcct cctcatcacc atcgccctag 121 ggaccaagac tgaatcctcc tcacggggac cttaccaccc ctcagagtgc tgcttcacct 181 acactaccta caagatcccg cgtcagcgga ttatggatta ctatgagacc aacagccagt 241 gctccaagcc cggaattgtc ttcatcacca aaaggggcca ttccgtctgt accaacccca 301 gtgacaagtg ggtccaggac tatatcaagg acatgaagga gaactgccca actttcttgt 361 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 421 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a