Construct: ORF ccsbBroad304_01521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001213.1_s304c1, BRDN0000409924
- Derived from:
- ccsbBroadEn_01521
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRSF1 (6426)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01521
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6426 | SRSF1 | serine and arginine rich sp... | NM_006924.5 | 100% | 100% | |
| 2 | human | 6426 | SRSF1 | serine and arginine rich sp... | NM_001078166.2 | 76% | 75% | (many diffs) |
| 3 | human | 6426 | SRSF1 | serine and arginine rich sp... | XR_429911.3 | 16.7% | 1_136del;881_4449del | |
| 4 | human | 6426 | SRSF1 | serine and arginine rich sp... | NR_034041.1 | 15.6% | 1_209del;760_959del;1154_4747del | |
| 5 | mouse | 110809 | Srsf1 | serine/arginine-rich splici... | NM_173374.4 | 94.4% | 100% | (many diffs) |
| 6 | mouse | 110809 | Srsf1 | serine/arginine-rich splici... | NM_001078167.2 | 71.1% | 75% | (many diffs) |
| 7 | mouse | 110809 | Srsf1 | serine/arginine-rich splici... | XR_388324.3 | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gggaggtggt gtgattcgtg gccccgcagg gaacaacgat tgccgcatct 121 acgtgggtaa cttacctcca gacatccgaa ccaaggacat tgaggacgtg ttctacaaat 181 acggcgctat ccgcgacatc gacctcaaga atcgccgcgg gggaccgccc ttcgccttcg 241 ttgagttcga ggacccgcga gacgcggaag acgcggtgta tggtcgcgac ggctatgatt 301 acgatgggta ccgtctgcgg gtggagtttc ctcgaagcgg ccgtggaaca ggccgaggcg 361 gcggcggggg tggaggtggc ggagctcccc gaggtcgcta tggcccccca tccaggcggt 421 ctgaaaacag agtggttgtc tctggactgc ctccaagtgg aagttggcag gatttaaagg 481 atcacatgcg tgaagcaggt gatgtatgtt atgctgatgt ttaccgagat ggcactggtg 541 tcgtggagtt tgtacggaaa gaagatatga cctatgcagt tcgaaaactg gataacacta 601 agtttagatc tcatgaggga gaaactgcct acatccgggt taaagttgat gggcccagaa 661 gtccaagtta tggaagatct cgatctcgaa gccgtagtcg tagcagaagc cgtagcagaa 721 gcaacagcag gagtcgcagt tactccccaa ggagaagcag aggatcacca cgctattctc 781 cccgtcatag cagatctcgc tctcgtacat acccaacttt cttgtacaaa gtggttggta 841 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 901 ggtggcgcgt taagtcgaca atcaacctct ggatta