Construct: ORF ccsbBroad304_01563
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013840.1_s304c1, BRDN0000401179
- Derived from:
- ccsbBroadEn_01563
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNCA (6622)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01563
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6622 | SNCA | synuclein alpha | NM_000345.4 | 100% | 100% | |
| 2 | human | 6622 | SNCA | synuclein alpha | NM_001146054.2 | 100% | 100% | |
| 3 | human | 6622 | SNCA | synuclein alpha | NM_001146055.2 | 100% | 100% | |
| 4 | human | 6622 | SNCA | synuclein alpha | XM_011532208.2 | 100% | 100% | |
| 5 | human | 6622 | SNCA | synuclein alpha | XM_017008562.1 | 100% | 100% | |
| 6 | human | 6622 | SNCA | synuclein alpha | XM_017008563.1 | 100% | 100% | |
| 7 | human | 6622 | SNCA | synuclein alpha | NM_007308.2 | 80% | 80% | 304_305ins84 |
| 8 | human | 6622 | SNCA | synuclein alpha | XM_011532203.1 | 71.2% | 52.2% | (many diffs) |
| 9 | human | 6622 | SNCA | synuclein alpha | XM_011532204.3 | 71.2% | 52.2% | (many diffs) |
| 10 | human | 6622 | SNCA | synuclein alpha | XM_011532205.2 | 71.2% | 52.2% | (many diffs) |
| 11 | human | 6622 | SNCA | synuclein alpha | XM_011532206.1 | 71.2% | 52.2% | (many diffs) |
| 12 | human | 6622 | SNCA | synuclein alpha | XM_011532207.1 | 71.2% | 52.2% | (many diffs) |
| 13 | mouse | 20617 | Snca | synuclein, alpha | NM_001042451.2 | 90.7% | 95% | (many diffs) |
| 14 | mouse | 20617 | Snca | synuclein, alpha | NM_009221.2 | 90.7% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tgtattcatg aaaggacttt caaaggccaa ggagggagtt gtggctgctg 121 ctgagaaaac caaacagggt gtggcagaag cagcaggaaa gacaaaagag ggtgttctct 181 atgtaggctc caaaaccaag gagggagtgg tgcatggtgt ggcaacagtg gctgagaaga 241 ccaaagagca agtgacaaat gttggaggag cagtggtgac gggtgtgaca gcagtagccc 301 agaagacagt ggagggagca gggagcattg cagcagccac tggctttgtc aaaaaggacc 361 agttgggcaa gaatgaagaa ggagccccac aggaaggaat tctggaagat atgcctgtgg 421 atcctgacaa tgaggcttat gaaatgcctt ctgaggaagg gtatcaagac tacgaacctg 481 aagcctaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 541 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 601 acctctggat ta