Construct: ORF ccsbBroad304_01607

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016776.1_s304c1, BRDN0000409270
Derived from:
ccsbBroadEn_01607
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STAT1 (6772)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01607
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6772 STAT1 signal transducer and activ... NM_139266.2 100% 100%
2 human 6772 STAT1 signal transducer and activ... NM_007315.3 94.8% 94.9% 2136_2250delinsG
3 human 6772 STAT1 signal transducer and activ... XM_006712718.1 94.8% 94.9% 2136_2250delinsG
4 human 6772 STAT1 signal transducer and activ... XM_017004783.2 94.6% 94.6% 1_6delATGTGG;2142_2256delinsG
5 human 6772 STAT1 signal transducer and activ... XR_001738915.2 44.4% 1_335del;2472_4804del
6 human 6772 STAT1 signal transducer and activ... XR_001738914.2 43.9% 1_393del;2530_4862del
7 mouse 20846 Stat1 signal transducer and activ... NM_001205314.1 82.2% 89% (many diffs)
8 mouse 20846 Stat1 signal transducer and activ... NM_009283.4 82.2% 89% (many diffs)
9 mouse 20846 Stat1 signal transducer and activ... XM_006495811.3 82.2% 89% (many diffs)
10 mouse 20846 Stat1 signal transducer and activ... XM_017319463.1 82.2% 89% (many diffs)
11 mouse 20846 Stat1 signal transducer and activ... XM_017319464.1 82.2% 89% (many diffs)
12 mouse 20846 Stat1 signal transducer and activ... NM_001205313.1 81.5% 88.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2202
ORF length:
2136
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tcagtggtac gaacttcagc agcttgactc aaaattcctg gagcaggttc
121accagcttta tgatgacagt tttcccatgg aaatcagaca gtacctggca cagtggttag
181aaaagcaaga ctgggagcac gctgccaatg atgtttcatt tgccaccatc cgttttcatg
241acctcctgtc acagctggat gatcaatata gtcgcttttc tttggagaat aacttcttgc
301tacagcataa cataaggaaa agcaagcgta atcttcagga taattttcag gaagacccaa
361tccagatgtc tatgatcatt tacagctgtc tgaaggaaga aaggaaaatt ctggaaaacg
421cccagagatt taatcaggct cagtcgggga atattcagag cacagtgatg ttagacaaac
481agaaagagct tgacagtaaa gtcagaaatg tgaaggacaa ggttatgtgt atagagcatg
541aaatcaagag cctggaagat ttacaagatg aatatgactt caaatgcaaa accttgcaga
601acagagaaca cgagaccaat ggtgtggcaa agagtgatca gaaacaagaa cagctgttac
661tcaagaagat gtatttaatg cttgacaata agagaaagga agtagttcac aaaataatag
721agttgctgaa tgtcactgaa cttacccaga atgccctgat taatgatgaa ctagtggagt
781ggaagcggag acagcagagc gcctgtattg gggggccgcc caatgcttgc ttggatcagc
841tgcagaactg gttcactata gttgcggaga gtctgcagca agttcggcag cagcttaaaa
901agttggagga attggaacag aaatacacct acgaacatga ccctatcaca aaaaacaaac
961aagtgttatg ggaccgcacc ttcagtcttt tccagcagct cattcagagc tcgtttgtgg
1021tggaaagaca gccctgcatg ccaacgcacc ctcagaggcc gctggtcttg aagacagggg
1081tccagttcac tgtgaagttg agactgttgg tgaaattgca agagctgaat tataatttga
1141aagtcaaagt cttatttgat aaagatgtga atgagagaaa tacagtaaaa ggatttagga
1201agttcaacat tttgggcacg cacacaaaag tgatgaacat ggaggagtcc accaatggca
1261gtctggcggc tgaatttcgg cacctgcaat tgaaagaaca gaaaaatgct ggcaccagaa
1321cgaatgaggg tcctctcatc gttactgaag agcttcactc ccttagtttt gaaacccaat
1381tgtgccagcc tggtttggta attgacctcg agacgacctc tctgcccgtt gtggtgatct
1441ccaacgtcag ccagctcccg agcggttggg cctccatcct ttggtacaac atgctggtgg
1501cggaacccag gaatctgtcc ttcttcctga ctccaccatg tgcacgatgg gctcagcttt
1561cagaagtgct gagttggcag ttttcttctg tcaccaaaag aggtcTCAAT GTGGACCAGC
1621TGAACATGTT GGGAGAGAAG CTTCTTGGTC CTAACGCCAG CCCCGATGGT CTCATTCCGT
1681GGACGAGGTT TTGTAAGGAA AATATAAATG ATAAAAATTT TCCCTTCTGG CTTTGGATTG
1741AAAGCATCCT AGAACTCATT AAAAAACACC TGCTCCCTCT CTGGAATGAT GGGTGCATCA
1801TGGGCTTCAT CAGCAAGGAG CGAGAGCGTG CCCTGTTGAA GGACCAGCAG CCGGGGACCT
1861TCCTGCTGCG GTTCAGTGAG AGCTCCCGGG AAGGGGCCAT CACATTCACA TGGGTGGAGC
1921GGTCCCAGAA CGGAGGCGAA CCTGACTTCC ATGCGGTTGA ACCCTACACG AAGAAAGAAC
1981TTTCTGCTGT TACTTTCCCT GACATCATTC GCAATTACAA AGTCATGGCT GCTGAGAATA
2041TTCCTGAGAA TCCCCTGAAG TATCTGTATC CAAATATTGA CAAAGACCAT GCCTTTGGAA
2101AGTATTACTC CAGGCCAAAG GAAGCACCAG AGCCAATGGA ACTTGATGGC CCTAAAGGAA
2161CTGGATATAT CAAGACTGAG TTGATTTCTG TGTCTGAAGT GTACCCAACT TTCTTGTACa
2221aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2281ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)