Construct: ORF ccsbBroad304_01611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017243.1_s304c1, BRDN0000404630
- Derived from:
- ccsbBroadEn_01611
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- STAT6 (6778)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01611
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6778 | STAT6 | signal transducer and activ... | NM_001178078.2 | 86.5% | 82.1% | (many diffs) |
| 2 | human | 6778 | STAT6 | signal transducer and activ... | NM_001178079.2 | 86.5% | 82.1% | (many diffs) |
| 3 | human | 6778 | STAT6 | signal transducer and activ... | NM_003153.5 | 86.5% | 82.1% | (many diffs) |
| 4 | human | 6778 | STAT6 | signal transducer and activ... | XM_011538703.3 | 84.9% | 80.4% | (many diffs) |
| 5 | human | 6778 | STAT6 | signal transducer and activ... | XM_011538704.3 | 84.9% | 80.4% | (many diffs) |
| 6 | human | 6778 | STAT6 | signal transducer and activ... | XM_011538705.3 | 84.9% | 80.4% | (many diffs) |
| 7 | human | 6778 | STAT6 | signal transducer and activ... | XM_011538707.3 | 84.9% | 80.4% | (many diffs) |
| 8 | human | 6778 | STAT6 | signal transducer and activ... | NM_001178080.2 | 74.5% | 69.2% | (many diffs) |
| 9 | human | 6778 | STAT6 | signal transducer and activ... | NM_001178081.2 | 74.5% | 69.2% | (many diffs) |
| 10 | human | 6778 | STAT6 | signal transducer and activ... | XM_011538708.3 | 73.1% | 67.8% | (many diffs) |
| 11 | human | 6778 | STAT6 | signal transducer and activ... | NR_033659.2 | 56.7% | (many diffs) | |
| 12 | human | 114771 | PGLYRP3 | peptidoglycan recognition p... | XM_011509120.2 | 32.4% | 3.7% | (many diffs) |
| 13 | human | 114771 | PGLYRP3 | peptidoglycan recognition p... | NM_052891.2 | 32.1% | 3.8% | (many diffs) |
| 14 | human | 114771 | PGLYRP3 | peptidoglycan recognition p... | XM_011509119.1 | 32.1% | 3.8% | (many diffs) |
| 15 | mouse | 20852 | Stat6 | signal transducer and activ... | NM_009284.2 | 76.3% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2208
- ORF length:
- 2142
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctgtggggt ctggtctcca agatgccccc agaaaaagtg cagcggctct 121 atgtcgactt tccccaacac ctgcggcatc ttctgggtga ctggctggag agccagccct 181 gggagttcct ggtcggctcc gacgccttct gctgcaactt ggctagtgcc ctactttcag 241 acactgtcca gcaccttcag gcctcggtgg gagagcaggg ggaggggagc accatcttgc 301 aacacatcag cacccttgag agcatatatc agagggaccc cctgaagctg gtggccactt 361 tcagacaaat acttcaagga gagaaaaaag ctgttatgga acagttccgc cacttgccaa 421 tgcctttcca ctggaagcag gaagaactca agtttaagac aggcttgcgg aggctgcagc 481 accgagtagg ggagatccac cttctccgag aagccctgca gaagggggct gaggctggcc 541 aagtgtctct gcacagcttg atagaaactc ctgctaatgg gactgggcca agtgaggccc 601 tggccatgct actgcaggag accactggag agctagaggc agccaaagcc ctagtgctga 661 agaggatcca gatttggaaa cggcagcagc agctggcagg gaatggcgca ccgtttgagg 721 agagcctggc cccactccag gagaggtgtg aaagcctggt ggacatttat tcccagctac 781 agcaggaggt aggggcggct ggtggggagc ttgagcccaa gacccgggca tcgctgactg 841 gccggctgga tgaagtcctg agaaccctcg tcaccagttg cttcctggtg gagaagcagc 901 ccccccaggt actgaagact cagaccaagt tccaggctgg agttcgattc ctgttgggct 961 tgaggttcct gggggcccca gccaagcctc cgctggtcag ggccgacatg gtgacagaga 1021 agcaggcgcg ggagctgagt gtgcctcagg gtcctggggc tggagcagaa agcactggag 1081 aaatcatcaa caacactgtg cccttggaga acagcattcc tgggaactgc tgctctgccc 1141 tgttcaagaa cctgcttctc aagaagatca agcggtgtga gcggaagggc actgagtctg 1201 tcacagagga gaagtgcgct gtgctcttct ctgccagctt cacacttggc cccggcaaac 1261 tccccatcca gctccaggcc ctgtctctgc ccctggtggt catcgtccat ggcaaccaag 1321 acaacaatgc caaagccact atcctgtggg acaatgcctt ctctgagatg gaccgcgtgc 1381 cctttgtggt ggctgagcgg gtgccctggg agaagatgtg tgaaactctg aacctgaagt 1441 tcatggctga ggtggggacc aaccgggggc tgctcccaga gcacttcctc ttcctggccc 1501 agaagatctt caatgacaac agcctcagta tggaggcctt ccagcaccgt tctgtgtcct 1561 ggtcgcagtt caacaaggag atcctgctgg gccgtggctt caccttttgg cagtggtttg 1621 atggtgtcct ggacctcacc aaacgctgtc tccggagcta ctggtctgac cggctgatca 1681 ttggcttcat cagcaaacag tacgttacta gccttcttct caatgagccc gacggaacct 1741 ttctcctccg cttcagcgac tcagagattg ggggcatcac cattgcccat gtcatccggg 1801 gccaggatgg ctctccacag atagagaaca tccagccatt ctctgccaaa gacctgtcca 1861 ttcgctcact gggggaccga atccgggatc ttgctcagct caaaaatctc tatcccaaga 1921 agcccaagga tgaggctttc cggagccact acaagcctga acagatgggt aaggatggca 1981 ggggttatgt cccagctacc atcaagatga ccgtggaaag ggaccaacca cttcctaccc 2041 cagagctcca gatgcctacc atggtgcctt cttatgacct tggaatggcc cctgattcct 2101 ccatgagcat gcagcttggc ccagatatgg tgccccaggt gtacccacca cacTCCTATG 2161 CCATCCAGAA GGGTCACTGT CGCCCAGGTA TATTCAGCCA CTTCTTCTGA AAGAAGAGAC 2221 CTGCCTGGAC CCTCAACATC CAGTGATGCC CAGGAAGGTT TGCCCCAACA TCATCAAACG 2281 ATCTGCTTGG GAAGCCAGAG AGACACACTG CCCTAAAATG AACCTCCCAG CCAAATATGT 2341 CATCATCATC CACACCGCTG GCACAAGCTG CACTGTATCC ACAGACTGCC AGACTGTCGT 2401 CCGAAACATA CAGTCCTTTC ACATGGACAC ACGGAACTTT TGTGACATTG GATATCACTT 2461 CCTGGTGGGC CAGGATGGTG GCGTGTATGA AGGGGTTGGA TGGCACATCC AAGGCTCTCA 2521 CACTTATGGA TTCAACGATA TTGCCCTAGG AATTGCCTTC ATCGGCTACT TTGTAGAAAA 2581 GCCTCCAAAT GCTGCAGCGC TGGAGGCGGC CCAGGACCTG ATCCAGTGTG CCGTGGTTGA 2641 GGGGTACCTG ACTCCAAACT ACCTGCTGAT GGGCCACAGT GACGTGGTCA ACATCCTGTC 2701 CCCTGGGCAG GCTTTGTATA ACATCATCAG CACCTGGCCT CATTTCAAGC ACTGCCCAAC 2761 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 2821 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2881 ATCTTGTGGA AAGGACGATC TGTTCCAACG TTATGTCGCC AAACGCGTTA AGTCGACAAT 2941 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT 3001 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTGTATCA TGCCATGCAA TGC