Construct: ORF ccsbBroad304_01618

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009917.1_s304c1, BRDN0000410215
Derived from:
ccsbBroadEn_01618
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STXBP1 (6812)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01618
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6812 STXBP1 syntaxin binding protein 1 NM_001032221.5 100% 100%
2 human 6812 STXBP1 syntaxin binding protein 1 NM_001374306.1 99.4% 99.3% 325_326insCTTGTCCAG
3 human 6812 STXBP1 syntaxin binding protein 1 NM_001374309.1 97.6% 97.6% 0_1ins42
4 human 6812 STXBP1 syntaxin binding protein 1 NM_001374310.1 97.6% 97.6% 0_1ins42
5 human 6812 STXBP1 syntaxin binding protein 1 NM_001374311.1 97.6% 97.6% 0_1ins42
6 human 6812 STXBP1 syntaxin binding protein 1 NM_001374312.1 97.6% 97.6% 0_1ins42
7 human 6812 STXBP1 syntaxin binding protein 1 NM_001374313.1 96.1% 95.7% (many diffs)
8 human 6812 STXBP1 syntaxin binding protein 1 NM_003165.5 96% 95.8% (many diffs)
9 human 6812 STXBP1 syntaxin binding protein 1 NM_001374314.1 96% 94.9% (many diffs)
10 human 6812 STXBP1 syntaxin binding protein 1 NM_001374307.1 93.7% 93.5% (many diffs)
11 human 6812 STXBP1 syntaxin binding protein 1 NM_001374308.1 93.7% 93.5% (many diffs)
12 human 6812 STXBP1 syntaxin binding protein 1 NM_001374315.1 90.1% 89.9% (many diffs)
13 mouse 20910 Stxbp1 syntaxin binding protein 1 NM_009295.2 90.8% 100% (many diffs)
14 mouse 20910 Stxbp1 syntaxin binding protein 1 NM_001113569.1 87.4% 95.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1848
ORF length:
1782
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccccattggc ctcaaagctg ttgtcggaga gaagattatg catgatgtga
121taaagaaggt caagaagaag ggggaatgga aggtgctggt ggtggatcag ttaagcatga
181ggatgctgtc ctcctgctgc aagatgacag acatcatgac cgagggcata acgattgtgg
241aagatatcaa taagcgcaga gagccgctcc ccagcctgga ggctgtgtat ctcatcactc
301catccgagaa gtccgtccac tctctcatca gtgactttaa ggacccgccg actgctaaat
361accgggctgc acacgtcttc ttcactgact cttgtccaga tgccctgttt aatgaactgg
421taaaatcccg agcagccaaa gtcatcaaaa ctctgacgga aatcaatatt gcatttctcc
481cgtatgaatc ccaggtctat tccttggact ctgctgactc tttccaaagc ttctacagtc
541cccacaaggc tcagatgaag aatcctatac tggagcgcct ggcagagcag atcgcgaccc
601tttgtgccac cctgaaggag tacccggctg tgcggtatcg gggggaatac aaggacaatg
661ccctgctggc tcagctaatc caggacaagc tcgatgccta taaagctgat gatccaacaa
721tgggggaggg cccagacaag gcacgctccc agctcctgat cctggatcga ggctttgacc
781ccagctcccc tgtgctccat gaattgactt ttcaggctat gagttatgat ctgctgccta
841tcgaaaatga tgtatacaag tatgagacca gcggcatcgg ggaggcacgg gtgaaggagg
901tgctcctgga cgaggacgac gacctgtgga tagcactgcg ccacaagcac atcgcagagg
961tgtcccagga agtcacccgg tctctgaaag atttttcttc tagcaagaga atgaatactg
1021gagagaagac caccatgcgg gacctgtccc agatgctgaa gaagatgcct cagtaccaga
1081aagagctcag caagtactcc acccacctgc accttgctga ggactgtatg aagcattacc
1141aaggcaccgt agacaaactc tgccgagtgg agcaggacct ggccatgggc acagatgctg
1201agggagagaa gatcaaggac cctatgcgag ccatcgtccc cattctgctg gatgccaatg
1261tcagcactta tgacaaaatc cgcatcatcc ttctctacat ctttttgaag aatggcatca
1321cggaggaaaa cctgaacaaa ctgatccagc acgcccagat acccccggag gatagtgaga
1381tcatcaccaa catggctcac ctcggcgtgc ccatcgtcac cgattccacg ctgcgtcgcc
1441ggagcaagcc ggagcggaag gaacgcatca gcgagcagac ctaccagctc tcacggtgga
1501ctccgattat caaggacatc atggaggaca ctattgagga caaacttgac accaaacact
1561acccttatat ctctacccgt tcctctgcct ccttcagcac caccgccgtc agcgcccgct
1621atgggcactg gcataagaac aaggccccag gcgagtaccg cagtggcccc cgcctcatca
1681ttttcatcct tgggggtgtg agcctgaatg agatgcgctg cgcctacgag gtgacccagg
1741ccaacggaaa gtgggaggtg ctgataggat ccacacacat cctcacccca cagaaactgc
1801tggacacact gaagaaactg aataaaacag atgaagaaat aagcagttac ccaactttct
1861tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1921aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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