Construct: ORF ccsbBroad304_01625

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005667.1_s304c1, BRDN0000411099
Derived from:
ccsbBroadEn_01625
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SURF4 (6836)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01625
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6836 SURF4 surfeit 4 NM_033161.4 100% 100%
2 human 6836 SURF4 surfeit 4 XM_011518943.1 96.2% 94.4% (many diffs)
3 human 6836 SURF4 surfeit 4 XM_011518944.2 96.2% 94.4% (many diffs)
4 human 6836 SURF4 surfeit 4 NM_001280788.1 94.1% 94% 0_1ins46;2delT
5 human 6836 SURF4 surfeit 4 NM_001280790.1 84% 84% 0_1ins129
6 human 6836 SURF4 surfeit 4 NM_001280791.1 84% 84% 0_1ins129
7 human 6836 SURF4 surfeit 4 XM_011518945.3 73.6% 32.3% (many diffs)
8 human 6836 SURF4 surfeit 4 NM_001280789.2 58.9% 58.7% (many diffs)
9 human 6836 SURF4 surfeit 4 NM_001280793.1 56.5% 56.5% 0_1ins351
10 human 6836 SURF4 surfeit 4 NM_001280792.1 47.5% 44.6% 356_357ins187;384_385ins236
11 human 6836 SURF4 surfeit 4 XM_017015062.1 40.7% 34% (many diffs)
12 mouse 20932 Surf4 surfeit gene 4 NM_011512.3 90.2% 99.2% (many diffs)
13 mouse 20932 Surf4 surfeit gene 4 XM_006497828.3 75.3% 83.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
873
ORF length:
807
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg ccagaacgac ctgatgggca cggccgagga cttcgccgac cagttcctcc
121gtgtcacaaa gcagtacctg ccccacgtgg cgcgcctctg tctgatcagc accttcctgg
181aggacggcat ccgtatgtgg ttccagtgga gcgagcagcg cgactacatc gacaccacct
241ggaactgcgg ctacctgctg gcctcgtcct tcgtcttcct caacttgctg ggacagctga
301ctggctgcgt cctggtgttg agcaggaact tcgtgcagta cgcctgcttc gggctctttg
361gaatcatagc tctgcagacg attgcctaca gcattttatg ggacttgaag tttttgatga
421ggaacctggc cctgggagga ggcctgttgc tgctcctagc agaatcccgt tctgaaggga
481agagcatgtt tgcgggcgtc cccaccatgc gtgagagctc ccccaaacag tacatgcagc
541tcggaggcag ggtcttgctg gttctgatgt tcatgaccct ccttcacttt gacgccagct
601tcttttctat tgtccagaac atcgtgggca cagctctgat gattttagtg gccattggtt
661ttaaaaccaa gctggctgct ttgactcttg ttgtgtggct ctttgccatc aacgtatatt
721tcaacgcctt ctggaccatt ccagtctaca agcccatgca tgacttcctg aaatacgact
781tcttccagac catgtcggtg attgggggct tgctcctggt ggtggccctg ggccctgggg
841gtgtctccat ggatgagaag aagaaggagt ggtacccaac tttcttgtac aaagtggttg
901gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
961accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)