Transcript: Human NM_001280792.1

Homo sapiens surfeit 4 (SURF4), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-05-21
Taxon:
Homo sapiens (human)
Gene:
SURF4 (6836)
Length:
2881
CDS:
198..584

Additional Resources:

NCBI RefSeq record:
NM_001280792.1
NBCI Gene record:
SURF4 (6836)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001280792.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000153266 GCTCTTTGCCATCAACGTATA pLKO.1 643 3UTR 100% 10.800 15.120 N SURF4 n/a
2 TRCN0000119385 CTTCCTGAAATACGACTTCTT pLKO.1 709 3UTR 100% 4.950 6.930 N Surf4 n/a
3 TRCN0000152319 CATGCATGACTTCCTGAAATA pLKO.1 700 3UTR 100% 13.200 9.240 N SURF4 n/a
4 TRCN0000151853 CCCAAAGAATGATGCAGTATT pLKO.1 1375 3UTR 100% 13.200 9.240 N SURF4 n/a
5 TRCN0000154091 CCAGTGGCTTTGATGTTGTTT pLKO.1 1307 3UTR 100% 5.625 3.938 N SURF4 n/a
6 TRCN0000158134 CTTCGGGCTCTTTGGAATCAT pLKO.1 479 CDS 100% 5.625 3.938 N SURF4 n/a
7 TRCN0000158166 CAGACGATTGCCTACAGCATT pLKO.1 507 CDS 100% 4.950 3.465 N SURF4 n/a
8 TRCN0000158412 CCCATGCATGACTTCCTGAAA pLKO.1 698 3UTR 100% 4.950 3.465 N SURF4 n/a
9 TRCN0000157961 CTGGCTGCTTTGACTCTTGTT pLKO.1 617 3UTR 100% 4.950 3.465 N SURF4 n/a
10 TRCN0000153161 GCACCATAGTAAATGCCACAA pLKO.1 2035 3UTR 100% 4.050 2.835 N SURF4 n/a
11 TRCN0000157847 CATTCCAGTCTACAAGCCCAT pLKO.1 682 3UTR 100% 2.160 1.512 N SURF4 n/a
12 TRCN0000119384 AGCAGGAACTTCGTGCAGTAT pLKO.1 453 CDS 100% 4.950 6.930 N Surf4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001280792.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01625 pDONR223 100% 47.5% 44.6% None 356_357ins187;384_385ins236 n/a
2 ccsbBroad304_01625 pLX_304 0% 47.5% 44.6% V5 356_357ins187;384_385ins236 n/a
3 TRCN0000481177 CCTCAATGGGATATCTACTCTCAG pLX_317 53.3% 47.5% 44.6% V5 356_357ins187;384_385ins236 n/a
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