Construct: ORF ccsbBroad304_01654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013218.1_s304c1, BRDN0000403696
- Derived from:
- ccsbBroadEn_01654
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCTA (6988)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01654
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6988 | TCTA | T cell leukemia translocati... | NM_022171.2 | 100% | 100% | |
| 2 | mouse | 102791 | Tcta | T cell leukemia translocati... | NM_001311156.1 | 86.4% | 89.6% | (many diffs) |
| 3 | mouse | 102791 | Tcta | T cell leukemia translocati... | NM_133986.2 | 75.1% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagtcctgg tctgggcagg ccttgcaggc tctgccggcc acggtgctgg 121 gcgcgctggg cagcgagttc ttgcgggagt gggaggcgca ggacatgcgc gtgaccctct 181 tcaagctgct gctgctgtgg ttggtgttaa gtctcctggg catccagctg gcgtgggggt 241 tctacgggaa tacagtgacc gggttgtatc accgtccagg tctgggtggt cagaatggat 301 ccacgcctga tggctccacg catttccctt cgtgggaaat ggcagcaaac gaacctctca 361 aaacccacag agaataccca actttcttgt acaaagtggt tggtaagcct atccctaacc 421 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 481 cgacaatcaa cctctggatt a