Construct: ORF ccsbBroad304_01702
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007126.1_s304c1, BRDN0000407446
- Derived from:
- ccsbBroadEn_01702
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPM2 (7169)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01702
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7169 | TPM2 | tropomyosin 2 | NM_213674.1 | 100% | 100% | |
| 2 | human | 7169 | TPM2 | tropomyosin 2 | NM_001301226.2 | 94.7% | 94.7% | (many diffs) |
| 3 | human | 7169 | TPM2 | tropomyosin 2 | NM_001301227.1 | 93.3% | 92.6% | (many diffs) |
| 4 | human | 7169 | TPM2 | tropomyosin 2 | XM_017015090.2 | 93.3% | 92.6% | (many diffs) |
| 5 | human | 7169 | TPM2 | tropomyosin 2 | NM_003289.3 | 88.3% | 87.3% | (many diffs) |
| 6 | human | 7169 | TPM2 | tropomyosin 2 | XM_017015091.2 | 88.3% | 87.3% | (many diffs) |
| 7 | human | 7169 | TPM2 | tropomyosin 2 | XM_017015087.2 | 77.9% | 69.3% | (many diffs) |
| 8 | human | 7169 | TPM2 | tropomyosin 2 | XM_017015088.2 | 77.9% | 69.3% | (many diffs) |
| 9 | human | 7169 | TPM2 | tropomyosin 2 | XM_017015092.2 | 75% | 75% | 639_640ins213 |
| 10 | human | 7169 | TPM2 | tropomyosin 2 | XM_017015093.2 | 75% | 75% | 639_640ins213 |
| 11 | mouse | 22004 | Tpm2 | tropomyosin 2, beta | NM_001277876.1 | 92.9% | 99.2% | (many diffs) |
| 12 | mouse | 22004 | Tpm2 | tropomyosin 2, beta | NM_001277875.1 | 88.1% | 94.3% | (many diffs) |
| 13 | mouse | 22004 | Tpm2 | tropomyosin 2, beta | XM_006537780.1 | 86.7% | 91.9% | (many diffs) |
| 14 | mouse | 22004 | Tpm2 | tropomyosin 2, beta | NM_009416.4 | 82.1% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgccatcaag aagaagatgc agatgctgaa gctggacaag gagaacgcca 121 tcgaccgcgc cgagcaggcc gaagccgaca agaagcaagc tgaggaccgc tgcaagcagc 181 tggaggagga gcagcaggcc ctccagaaga agctgaaggg gacagaggat gaggtggaaa 241 agtattctga atccgtgaag gaggcccagg agaaactgga gcaggccgag aagaaggcca 301 ctgatgctga ggcagatgtg gcctccctga accgccgcat tcagctggtt gaggaggagc 361 tggaccgggc ccaggagcgc ctggctacag ccctgcagaa gctggaggag gccgagaagg 421 cggctgatga gagcgagaga ggaatgaagg tcatcgaaaa ccgggccatg aaggatgagg 481 agaagatgga actgcaggag atgcagctga aggaggccaa gcacatcgct gaggattcag 541 accgcaaata tgaagaggtg gccaggaagc tggtgatcct ggaaggagag ctggagcgct 601 cggaggagag ggctgaggtg gccgagagcc gagccagaca gctggaggag gaacttcgaa 661 ccatggacca ggccctcaag tccctgatgg cctcagagga ggagtattcc accaaagaag 721 ataaatatga agaggagatc aaactgttgg aggagaagct gaaggaggct gagacccgag 781 cagagtttgc cgagaggtct gtggcaaagt tggagaaaac catcgatgac ctagaagaga 841 ccttggccag tgccaaggag gagaacgtcg agattcacca gaccttggac cagaccctgc 901 tggaactcaa caacctgtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 961 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1021 agtcgacaat caacctctgg atta