Construct: ORF ccsbBroad304_01757

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010357.1_s304c1, BRDN0000403212
Derived from:
ccsbBroadEn_01757
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UQCRC2 (7385)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01757
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7385 UQCRC2 ubiquinol-cytochrome c redu... NM_003366.4 100% 100%
2 mouse 67003 Uqcrc2 ubiquinol cytochrome c redu... NM_025899.2 86% 85.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1425
ORF length:
1359
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gctactaacc agagccggct ctttctcgag attttattcc ctcaaagttg
121cccccaaagt taaagccaca gctgcgcctg caggagcacc gccacaacct caggaccttg
181agtttaccaa gttaccaaat ggcttggtga ttgcttcttt ggaaaactat tctcctgtat
241caagaattgg tttgttcatt aaagcaggca gtagatatga ggacttcagc aatttaggaa
301ccacccattt gctgcgtctt acatccagtc tgacgacaaa aggagcttca tctttcaaga
361taacccgtgg aattgaagca gttggtggca aattaagtgt gaccgcaaca agggaaaaca
421tggcttatac tgtggaatgc ctgcggggtg atgttgatat tctaatggag ttcctgctca
481atgtcaccac agcaccagaa tttcgtcgtt gggaagtagc tgaccttcag cctcagctaa
541agattgacaa agctgtggcc tttcagaatc cgcagactca tgtcattgaa aatttgcatg
601cagcagctta ccggaatgcc ttggctaatc ccttgtattg tcctgactat aggattggaa
661aagtgacatc agaggagtta cattacttcg ttcagaacca tttcacaagt gcaagaatgg
721ctttgattgg acttggtgtg agtcatcctg ttctaaagca agttgctgaa cagtttctca
781acatgagggg tgggcttggt ttatctggtg caaaggccaa ctaccgtgga ggtgaaatcc
841gagaacagaa tggagacagt cttgtccatg ctgcttttgt agcagaaagt gctgtcgcgg
901gaagtgcaga ggcaaatgca tttagtgttc ttcagcatgt cctcggtgct gggccacatg
961tcaagagggg cagcaacacc accagccatc tgcaccaggc tgttgccaag gcaactcagc
1021agccatttga tgtttctgca tttaatgcca gttactcaga ttctggactc tttgggattt
1081atactatctc ccaggccaca gctgctggag atgttatcaa ggctgcctat aatcaagtaa
1141aaacaatagc tcaaggaaac ctttccaaca cagatgtcca agctgccaag aacaagctga
1201aagctggata cctaatgtca gtggagtctt ctgagtgttt cctggaagaa gtcgggtccc
1261aggctctagt tgctggttct tacatgccac catccacagt ccttcagcag attgattcag
1321tggctaatgc tgatatcata aatgcggcaa agaagtttgt ttctggccag aagtcaatgg
1381cagcaagtgg aaatttggga catacacctt ttgttgatga gttgtaccca actttcttgt
1441acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1501gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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