Construct: ORF ccsbBroad304_01759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002929.1_s304c1, BRDN0000409398
- Derived from:
- ccsbBroadEn_01759
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UROS (7390)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01759
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7390 | UROS | uroporphyrinogen III synthase | NM_000375.3 | 100% | 100% | |
| 2 | human | 7390 | UROS | uroporphyrinogen III synthase | XM_024448154.1 | 100% | 100% | |
| 3 | human | 7390 | UROS | uroporphyrinogen III synthase | NM_001324036.2 | 90.7% | 90.7% | 660_740del |
| 4 | human | 7390 | UROS | uroporphyrinogen III synthase | XM_017016611.2 | 90.7% | 90.7% | 660_740del |
| 5 | human | 7390 | UROS | uroporphyrinogen III synthase | NM_001324038.2 | 89.8% | 89.8% | 394_395ins81 |
| 6 | human | 7390 | UROS | uroporphyrinogen III synthase | XM_017016612.2 | 86.7% | 82.4% | (many diffs) |
| 7 | human | 7390 | UROS | uroporphyrinogen III synthase | XM_011540127.2 | 86.5% | 83.8% | (many diffs) |
| 8 | human | 7390 | UROS | uroporphyrinogen III synthase | XM_005270140.5 | 83.7% | 83% | (many diffs) |
| 9 | human | 7390 | UROS | uroporphyrinogen III synthase | NM_001324037.2 | 81.5% | 81.5% | 394_395ins81;579_659del |
| 10 | human | 7390 | UROS | uroporphyrinogen III synthase | XM_024448155.1 | 73.5% | 72.8% | (many diffs) |
| 11 | human | 7390 | UROS | uroporphyrinogen III synthase | XR_246103.3 | 61.9% | 1_123del;599_670del;991_1283del | |
| 12 | human | 7390 | UROS | uroporphyrinogen III synthase | NR_136678.2 | 56.4% | (many diffs) | |
| 13 | human | 7390 | UROS | uroporphyrinogen III synthase | XR_945810.2 | 52.5% | 1_123del;599_900del;1221_1513del | |
| 14 | human | 7390 | UROS | uroporphyrinogen III synthase | NR_136677.1 | 52% | (many diffs) | |
| 15 | human | 7390 | UROS | uroporphyrinogen III synthase | NM_001324039.1 | 51.5% | 50.1% | (many diffs) |
| 16 | human | 7390 | UROS | uroporphyrinogen III synthase | NR_136676.2 | 50.2% | 1_256del;916_1161del;1298_1582del | |
| 17 | human | 7390 | UROS | uroporphyrinogen III synthase | NR_136675.2 | 45.9% | 1_256del;318_319ins181;871_1155del | |
| 18 | human | 7390 | UROS | uroporphyrinogen III synthase | XR_002957010.1 | 32.7% | 1_64del;540_1814del;2135_2427del | |
| 19 | human | 7390 | UROS | uroporphyrinogen III synthase | XR_002957009.1 | 32.6% | (many diffs) | |
| 20 | human | 7390 | UROS | uroporphyrinogen III synthase | XR_001747196.2 | 31.3% | (many diffs) | |
| 21 | human | 7390 | UROS | uroporphyrinogen III synthase | XR_001747197.2 | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 861
- ORF length:
- 795
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggttctttta ctgaaggatg cgaaggaaga tgactgtggc caggatccgt 121 atatcaggga attaggatta tatggacttg aagccacttt gatccctgtt ttatcgtttg 181 agtttttgtc tcttcccagt ttctctgaga agctttctca tcctgaagat tacgggggac 241 tcatttttac cagccccaga gcagtggaag cagcagagtt atgtttggag caaaacaata 301 aaactgaagt ctgggaaagg tctctgaaag aaaaatggaa tgccaagtca gtgtatgtgg 361 ttggaaatgc tactgcttct ctagtgagta aaattggcct ggatacagaa ggagaaacct 421 gtggaaatgc agaaaagctt gcagaatata tttgttccag ggagtcctca gcactgcctc 481 ttctatttcc ctgtggaaac ctcaaaagag aaatcctgcc aaaagcgctc aaggacaaag 541 ggattgccat ggaaagcata actgtgtatc agacagttgc acacccagga atccaaggga 601 acctgaacag ctactattcc cagcaggggg ttccagccag catcacattt tttagtccct 661 ctggcctcac atacagtctc aagcacattc aggagttatc tggtgacaat atcgatcaaa 721 ttaagtttgc agccatcggc cccactacgg ctcgcgcgct ggccgcccag ggccttcctg 781 taagctgcac tgcagagagc cccacgccac aagccctggc cactggcatc aggaaggctc 841 tccagcccca tggctgctgc tgcccaactt tcttgtacaa agtggttggt aagcctatcc 901 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 961 ttaagtcgac aatcaacctc tggatta