Construct: ORF ccsbBroad304_01763

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014995.1_s304c1, BRDN0000411477
Derived from:
ccsbBroadEn_01763
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VCAM1 (7412)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01763
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7412 VCAM1 vascular cell adhesion mole... NM_001078.4 99.9% 100% 2208G>A
2 human 7412 VCAM1 vascular cell adhesion mole... NM_001199834.1 91.5% 91.6% 152_153ins186;2022G>A
3 human 7412 VCAM1 vascular cell adhesion mole... NM_080682.2 87.5% 87.4% 928_929ins276;1932G>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2283
ORF length:
2217
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc tgggaagatg gtcgtgatcc ttggagcctc aaatatactt tggataatgt
121ttgcagcttc tcaagctttt aaaatcgaga ccaccccaga atctagatat cttgctcaga
181ttggtgactc cgtctcattg acttgcagca ccacaggctg tgagtcccca tttttctctt
241ggagaaccca gatagatagt ccactgaatg ggaaggtgac gaatgagggg accacatcta
301cgctgacaat gaatcctgtt agttttggga acgaacactc ttacctgtgc acagcaactt
361gtgaatctag gaaattggaa aaaggaatcc aggtggagat ctactctttt cctaaggatc
421cagagattca tttgagtggc cctctggagg ctgggaagcc gatcacagtc aagtgttcag
481ttgctgatgt atacccattt gacaggctgg agatagactt actgaaagga gatcatctca
541tgaagagtca ggaatttctg gaggatgcag acaggaagtc cctggaaacc aagagtttgg
601aagtaacctt tactcctgtc attgaggata ttggaaaagt tcttgtttgc cgagctaaat
661tacacattga tgaaatggat tctgtgccca cagtaaggca ggctgtaaaa gaattgcaag
721tctacatatc acccaagaat acagttattt ctgtgaatcc atccacaaag ctgcaagaag
781gtggctctgt gaccatgacc tgttccagcg agggtctacc agctccagag attttctgga
841gtaagaaatt agataatggg aatctacagc acctttctgg aaatgcaact ctcaccttaa
901ttgctatgag gatggaagat tctggaattt atgtgtgtga aggagttaat ttgattggga
961aaaacagaaa agaggtggaa ttaattgttc aagagaaacc atttactgtt gagatctccc
1021ctggaccccg gattgctgct cagattggag actcagtcat gttgacatgt agtgtcatgg
1081gctgtgaatc cccatctttc tcctggagaa cccagataga cagccctctg agcgggaagg
1141tgaggagtga ggggaccaat tccacgctga ccctgagccc tgtgagtttt gagaacgaac
1201actcttatct gtgcacagtg acttgtggac ataagaaact ggaaaaggga atccaggtgg
1261agctctactc attccctaga gatccagaaa tcgagatgag tggtggcctc gtgaatggga
1321gctctgtcac tgtaagctgc aaggttccta gcgtgtaccc ccttgaccgg ctggagattg
1381aattacttaa gggggagact attctggaga atatagagtt tttggaggat acggatatga
1441aatctctaga gaacaaaagt ttggaaatga ccttcatccc taccattgaa gatactggaa
1501aagctcttgt ttgtcaggct aagttacata ttgatgacat ggaattcgaa cccaaacaaa
1561ggcagagtac gcaaacactt tatgtcaatg ttgcccccag agatacaacc gtcttggtca
1621gcccttcctc catcctggag gaaggcagtt ctgtgaatat gacatgcttg agccagggct
1681ttcctgctcc gaaaatcctg tggagcaggc agctccctaa cggggagcta cagcctcttt
1741ctgagaatgc aactctcacc ttaatttcta caaaaatgga agattctggg gtttatttat
1801gtgaaggaat taaccaggct ggaagaagca gaaaggaagt ggaattaatt atccaagtta
1861ctccaaaaga cataaaactt acagcttttc cttctgagag tgtcaaagaa ggagacactg
1921tcatcatctc ttgtacatgt ggaaatgttc cagaaacatg gataatcctg aagaaaaaag
1981cggagacagg agacacagta ctaaaatcta tagatggcgc ctataccatc cgaaaggccc
2041agttgaagga tgcgggagta tatgaatgtg aatctaaaaa caaagttggc tcacaattaa
2101gaagtttaac acttgatgtt caaggaagag aaaacaacaa agactatttt tctcctgagc
2161ttctcgtgct ctattttgca tcctccttaa taatacctgc cattggaatg ataatttact
2221ttgcaagaaa agccaacatg aaggggtcat atagtcttgt agaagcacag aaatcaaaag
2281tgtacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2341tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2401tctggatta

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