Construct: ORF ccsbBroad304_01775

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010196.1_s304c1, BRDN0000409833
Derived from:
ccsbBroadEn_01775
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF4H (7458)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01775
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7458 EIF4H eukaryotic translation init... NM_031992.2 100% 100%
2 human 7458 EIF4H eukaryotic translation init... NM_022170.2 91.9% 91.9% 409_468del
3 mouse 22384 Eif4h eukaryotic translation init... NM_001312867.1 90.7% 99.1% (many diffs)
4 mouse 22384 Eif4h eukaryotic translation init... NM_033561.2 83.4% 91.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
750
ORF length:
684
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggacttcgac acctacgacg atcgggccta cagcagcttc ggcggcggca
121gagggtcccg cggcagtgct ggtggccatg gttcccgtag ccagaaggag ttgcccacag
181agccccccta cacagcatac gtaggaaatc tacctttcaa tacggttcag ggcgacatag
241atgctatctt taaggatctc agcataagga gtgtacggct agtcagagac aaagacacag
301ataaatttaa aggattctgc tatgtagaat tcgatgaagt ggattccctt aaggaagcct
361tgacatacga tggtgcactg ttgggcgatc ggtcacttcg tgtggacatt gcagaaggca
421gaaaacaaga taaaggtggc tttggattca gaaaaggtgg accagatgac agaggcttca
481gggatgactt cttagggggc aggggaggta gtcgcccagg cgaccggcga acaggccccc
541ccatgggcag ccgcttcaga gatggccctc ccctccgtgg atccaacatg gatttcagag
601aacccacaga agaggaaaga gcacagagac cacgactcca gcttaaacct cgaacagtcg
661cgacgcccct caatcaagta gccaatccca actctgctat cttcgggggt gccaggccta
721gagaggaagt cgttcaaaag gagcaagaat gcccaacttt cttgtacaaa gtggttggta
781agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
841ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)